Vero cell line (derived from the normal kidney of adult monkeys) and human adenocarcinoma colon HT29 cells were purchased from the National Center for Cell Sciences, Pune, India. The cells were cultured under standard conditions in Dulbecco’s Modified Eagle Medium (DMEM), supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 U/mL of streptomycin in a humidified incubator set at 37°C with 5% CO2.17
Vero cell line
Manufactured by Cell Sciences
Sourced in India
The Vero cell line is a continuous cell line derived from the kidney of the African green monkey (Chlorocebus sabaeus). It is a well-established and widely used cell line in cell biology research and biomedical applications. The Vero cell line has a stable and consistent growth pattern, making it a reliable and reproducible model for various in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using vero cell line
1
Cell culture protocol for Vero and HT29
2
Evaluation of Crude Oil Bioremediation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chemicals such as silver nitrate, gelatin, and Bushnell Haas Broth were obtained from Hi-media (Mumbai, India) and used as received. Dichloromethane, ethyl acetate, MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide], fetal bovine serum (FBS), dulbecco’s modified eagle medium (DMEM), streptomycin, penicillin and dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich. Vero cell line (African green monkey kidney normal cell line) was obtained from National Center for Cell Sciences (NCCS), Pune, India. The crude oil sample was collected from India’s Oil and natural gas corporation (ONGC) in Karaikal, India (Parthipan et al., 2017 ).
3
Maintenance of Fibroblast and Vero Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Fibroblast cell line (MCCOY) and Vero cell line were obtained from the National Center for Cell Sciences (NCCS), Pune, India. Cells were maintained in the logarithmic growth phase in DMEM medium containing L-glutamine (2 mmol), sodium bicarbonate (g/l), glucose (4.5 g/l), HEPES (10 mmol) and sodium pyruvate (1.0 mmol). It was supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 100 μg/ml penicillin, and 100 μg/ml streptomycin in an air-humidified incubator with 5% CO2 at 37°C.
4
Culturing Human and Monkey Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human epidermoid carcinoma A431 cell line and kidney epithelial Vero cell line were purchased from the National Center for Cell Sciences (NCCS), Pune, India. The cell lines were subcultured in Dulbecco’s Modified Eagle Medium (DMEM)-F12 medium, which also contained 10% (v/v) fetal calf serum (FCS), sodium bicarbonate (NaHCO3) (1.5 g/L), and L-glutamine (2 mM).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!