miRNAs were extracted from the serum and tissues as described previously,21 with some modifications. One Step Prime Script miRNA cDNA (Perfect Real Time) Kit (TaKaRa Biotechnology, Dalian, People’s Republic of China) was used in the reverse transcription reaction and miRcute miRNA qPCR detection kit SuperReal PreMix Plus (SYBR) (Tiangen Biotech, Beijing, People’s Republic of China) was used for real-time quantitative PCR. Details of the materials are shown in the Supplementary materials.
One step prime script mirna cdna perfect real time kit
Manufactured by Takara Bio
Sourced in China
The One Step Prime Script miRNA cDNA (Perfect Real Time) Kit is a laboratory reagent designed for the reverse transcription and real-time PCR amplification of mature microRNA (miRNA) molecules. The kit provides the necessary components, including a reverse transcriptase enzyme and specific primers, to efficiently convert miRNA into complementary DNA (cDNA) in a single-step reaction, which can then be used for quantitative real-time PCR analysis.
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Lab products found in correlation
3 protocols using one step prime script mirna cdna perfect real time kit
1
miRNA Extraction and Quantification
2
miRNA Extraction and Quantification from Serum and Tissue
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MiRNA was extracted from serum and tissue samples as described previously45 (link) with some modifications. The One Step Prime Script miRNA cDNA (Perfect Real-Time) Kit (TAKARA Biotechnology Co., Ltd, Dalian, China) was used to generate cDNA and the miRcute miRNA qPCR detection kit (SYBR) (TIANGEN Biotech Co., Ltd, Beijing, China) was used for real-time PCR analysis with the primer 5′-CGCGTTCACCGTGGCTAAGTTCC-3′. The methods used have been described previously46 (link)47 (link).
3
miRNA Extraction and Quantification from Serum and Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The miRNA extracted method from the serum and tissue was used as described by the literature [29] (link) with some modifications. The reverse transcription reaction was used One Step Prime Script miRNA cDNA (Perfect Real Time) Kit (TAKARA Biotechnology Co., Ltd, Dalian, China) and Real-time PCR reaction was used miRcute miRNA qPCR detection kit (SYBR) (TIANGEN Biotech Co., Ltd, Beijing, China). The detailed materials were shown in the Supplementary Methods .
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