Molecular imager chemidoc xrs system image lab software v 4

A Proteome Profiler Mouse Cytokine Array Kit, Panel A (R&D system, Minneapolis, MN, USA) was employed for the profiling of chemokines, cytokines, and acute phase proteins following the LPS-induced inflammation and subsequent treatment with free DEX and DEX-LKs. This array allows for the detection of multiple molecules at the same time. The results were obtained using the manufacturer’s instructions. The protein array images were scanned and pixel density analyzed by Molecular Imager ChemiDoc XRS System + Image Lab Software v.4.1 (Bio-Rad, Hercules, CA, USA). The basal levels of plasma cytokines of non-treated mice were evaluated by the same array and used to normalize the data.

The Rat Cytokine array panel A (R&D system, Minneapolis, MN) was employed for the profiling of the chemokines and cytokines secreted upon CL and CSCL scaffolds implantation. This array allows the detection of 29 different molecules. We collected and quantified the proteins adsorbed on the scaffolds using the procedure described above (Bio-Rad). The results were obtained using the manufacturer’s instruction. The protein array images were scanned and pixel density analyzed by Molecular Imager ChemiDoc XRS System + Image Lab Software v.4.1 (BioRad). The experiments were performed in triplicate.