Cd34 hm34

Manufactured by BioLegend

CD34 (HM34) is a cell surface glycoprotein expressed on hematopoietic stem and progenitor cells. It functions as a marker for identifying and isolating these cell populations.

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2 protocols using cd34 hm34

Flow Cytometry and Microscopy Antibody Staining

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Antibodies used for flow cytometry and immunofluorescent microscopy included those against human CD1b (SN13, Biolegend), Siglec-7 (QA79, eBioscience), CD14 (HCD14, Biolegend), CD56 (HCD56, Biolegend), CD34 (HM34, Biolegend), CD3 (UCHT1, BD Biosciences), CD19 (HIB19, Biolegend), HLA-DR (L243, Biolegend), CD11c (3.9, Biolegend), CD123 (6H6, Biolegend), EEA-1 (ab2900, Abcam) and LAMP-1 (ab24170, Abcam). For the blocking of LDN5 cell activation and liposome binding, anti-CD1b (BCD1b.3) (36 (link)) and anti-Siglec-7 (S7.7, Biolegend) were used, respectively. C80 GMM was isolated as described (36 (link)). Ficoll Plaque plus (GE Healthcare) was used for the density gradient centrifugation to separate peripheral blood mononuclear cells. Human IFNγ ELISA kit (Biolegend) was used to measure human IFNγ.

Kawasaki N., Rillahan C.D., Cheng T.Y., Van Rhijn I., Macauley M.S., Moody D.B, & Paulson J.C. (2014). Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation. Journal of immunology (Baltimore, Md. : 1950), 193(4), 1560-1566.

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Surface Marker Analysis of Immune Cells

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For surface markers analysis, live cells were re-suspended in 1 × PBS and stained with anti-mouse CD45 (30-F11, Biolegend), CD11b (M1/70, R&D Systems), F4/80 (BM8, Biolegend), CD86 (GL-1, Biolegend), MHC-II (M5/114.15.2, Biolegend), CD117 (2B8, Biolegend), CD115 (AFS98, Biolegend), CD16/32 (93, Biolegend), CD34 (HM34, Biolegend), Sca1 (D7, Biolegend), SIRPα (P84, biolegend), Lin (Stem Cell), and APC-Cy7 Streptavidin (Biolegend) at 4°C for 30 min. The concentration at each antibody was used as the product protocol recommended. For intracellular cytokine staining, cells were fixed and permeabilized with Fixation and Permeabilization Kit (eBioscience) at room temperature for 40 min and labeled with anti-mouse CD206 (C068C2, Biolegend). Multicolor FACS analysis was performed on an LSR Fortessa Analyzer (BD Biosciences). All data analysis was performed using the flow cytometry analysis program FlowJo-V10.

Zhao C.C., Han Q.J., Ying H.Y., Gu X.X., Yang N., Li L.Y, & Zhang Q.Z. (2022). TNFSF15 facilitates differentiation and polarization of macrophages toward M1 phenotype to inhibit tumor growth. Oncoimmunology, 11(1), 2032918.

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