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6 protocols using propylene glycol

1

Extracellular Matrix Protein Analysis

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Glycine, phenazine methosulfate fluoride (PMSF), Bradford reagent, and Tris-HCl were purchased from Sigma (Sigma Chemical Co., USA). Carbopol 934, propylene glycol, gentamicin sulfate, l-hydroxyproline, and p-dimethyl-amino-benzaldehyde were obtained from Himedia (Himedia Pvt. Labs., India). bFGF, COL3A1, Smad-2, Smad-3, Smad-4, and Smad-7, and β-actin primary and secondary antibodies and BCIP- (5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt-) NBT reagents were procured from Santa Cruz (Santa Cruz, USA). PVDF membrane was from Millipore (Millipore Corp., USA) and Western Max-HRP-Chromogenic detection kit was purchased from Amresco (Amresco, USA). All other chemicals and reagents not mentioned were of the analytical grade.
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2

Dolutegravir Formulation Development

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Dolutegravir (MW, 419.38 Da, and 99.99%) was obtained as a gratis sample from Emcure Pharmaceuticals, Gandhinagar, India. Transcutol® P, Capryol® 90, Labrasol®, and Labrafil® M were purchased from Gattefosse, Saint-Priest, France, and were of USP-NF/EP quality. Dill oil (95.9%) and corn oil (92.3%) were gifted from Vicci Win Pharma, Ahmedabad, India. Castor oil (88.5%) was gifted from Gujarat Glycols, Ankhleswar, India. Polyethylene glycol (PEG) 400 (98%), butyl carbitol (92%), and propylene glycol (99%) were gifted from Himedia, Mumbai, India. Tween® 80 (97.3%), and Tween® 20 (97.3%) were received as gift samples from Finar Chemical, Ahmedabad, India. All other reagents/chemicals used in this study are of analytical grade.
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3

Transdermal Finasteride and Testosterone Formulation

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Chitosan (low molecular weight), sodium alginate, calcium chloride, acetic acid, sodium hydroxide, carbopol 934, methyl paraben, propyl paraben, propylene glycol, triethanolamine, finasteride (all were procured from Hi-Media laboratories, Mumbai, India), testosterone (Loba Chemie Pvt Ltd., Mumbai, India), and carboxymethylcellulose (Sigma-Aldrich) were used. All chemicals and solvents used were of analytical reagent grade.
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4

Formulation and Characterization of Lipid-Based Nanocarriers

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Lomustine and Capryol 90 was purchased from Aladdin, China. Span 20, 40, 80, Tween 20, 40, 60, 80, PEG 200, 400, propylene glycol and methyl orange were bought from Himedia Laboratories Pvt. Ltd. (India). Oleic acid was purchased from Central Drug House (P) Ltd. (CDH®), New Delhi (India). Olive oil was from Central Drug House (P) Ltd. Castor, and eucalyptus oils, almond oil and cod liver oil were from Wuhan Senwayer Century Chemical Co., Ltd, China. Tricetin from Quzhou Rundong Chemical Co., Ltd., China. Cromophor EL and transcutol P were from Shanghai Ruizheng chemical Tech Co., Ltd, China. Labrasol was from Gattefosse, USA. Methanol and cumin oil were purchased from Sigma-Aldrich, USA. Peppermint oil was from BAR-SUR-LOUP, France.
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5

Primaquine-Loaded Transdermal Drug Delivery

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Primaquine was procured as a gift sample from Indian Pharmaceutical Combine Association Limited laboratories (IPCA laboratories, Ratlam, India). Castor oil I.P. (Indian Pharmacopeia) was purchased from a chemist shop (manufactured by Search Creation Company). Transcutol P (diethylene glycol monoethyl ether), propylene glycol, Span 80, and hydroxypropyl methylcellulose (HPMC) were procured from Himedia Laboratories Pvt. Ltd., Delhi, India. Tween 80 was procured from Ramagundam Fertilizers And Chemicals Limited (RFCL Ltd.) Delhi, India. Solvents, water, and other chemicals were used of analytical grade (chemicals that meet specifications of American Chemical Society (ACS) are called reagent or analytical grade). The animal experimental studies were approved by IAEC (Institutional Animal Ethical Committee) as per the regulation committee for CPCSEA (Committee for Purpose of Control and Supervision of Experiments on Animals) Government of India and registration number of approval was IAEC/JU/47.
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6

Apremilast Topical Formulation Development

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Apremilast was received as a gift sample from Glenmark life Sciences Ltd, Ankleshwar, Gujarat. Sefsol 218, Sefsol 228, Transcutol P were collected from Gattefosse, France. Glyceryl Monostearate, Tween 80, propylene glycol, tween 20, oleic acid, olive oil, PEG 400, Isopropyl alcohol, n butanol were procured from Himedia Laboratories Pvt. Ltd. Stearic acid, palmitic acid, and myristic acid were obtained from Daejung Chemical (Cheongwon, Korea). Ultra-pure deionized water was used throughout the experiments. All other solvents and chemicals used were of analytical grades or higher.
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