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Griess reagent nitric oxide assay kit

Manufactured by Beyotime
Sourced in China, Puerto Rico

The Griess reagent nitric oxide assay kit is a laboratory equipment used to measure the concentration of nitric oxide (NO) in biological samples. It provides a colorimetric method for the quantitative determination of nitrite, a stable metabolite of NO. The kit includes the necessary reagents and instructions to perform the assay.

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2 protocols using griess reagent nitric oxide assay kit

1

Inflammatory Signaling Pathway Analysis

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Rabbit anti-mouse NF-κB65 and p38 MAPK antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse TNF-α, IL-1β and IL-6 ELISA kits were purchased from Quantikine, R&D Systems (Minneapolis, MN, USA). The Griess reagent nitric oxide assay kit was purchased from Beyotime Biotech (Jiangsu, China). The mouse IL-10 ELISA kit was obtained from Bender MedSystems (Vienna, Austria). Paraquat was purchased from Sigma.
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2

Nitric Oxide Production Measurement

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The cells were pre-treated with CGA 5 h before LTA stimulation for 24 h after which the supernatants were collected. The concentration of NO in the supernatants was measured using a Griess reagent Nitric Oxide Assay Kit (Beyotime Biotechnology, Shanghai, P. R. China, cat. S0023) via detection of the nitrite level as the nitrite level represents NO production.
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