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Tunel staining kit in situ cell death detection kit

Manufactured by Roche
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Sourced in United States

The TUNEL staining kit (In Situ Cell Death Detection Kit) is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in tissue samples. The kit utilizes the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method to identify DNA fragmentation, a hallmark of apoptosis. The kit provides the necessary reagents and protocols to perform this specialized staining procedure.

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Lab products found in correlation

L glutamine, by Thermo Fisher Scientific (2 mentions) Mem non essential amino acid, by Merck Group (2 mentions) Rabbit anti p erk, by Cell Signaling Technology (1 mentions) Anti rabbit and anti mouse secondary antibodies, by Jackson ImmunoResearch (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Lithium chloride (licl), by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

In Situ Cell Death Detection Kit (3572 citations) Cell Death Detection Kit (199 citations) TUNEL in situ cell death detection kit (67 citations) In situ cell death kit (48 citations) In Situ Death Detection Kit (21 citations) Situ Cell Death Detection Kit (16 citations) TUNEL cell death detection kit (8 citations) TUNEL staining in situ cell death detection kit (4 citations) In Situ Death Detection TUNEL Kit (4 citations) TUNEL staining detection kit (2 citations)

3 protocols using tunel staining kit in situ cell death detection kit

1

Grape Seed Proanthocyanidin Protects Pancreatic Cells

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Grape seed proanthocyanidin (purity exceeds 96%, Lot no. G050412) was purchased from Tianjin Jianfeng Natural Product R&D Co., Ltd. (Tianjin, China). STZ was purchased from Sigma. TUNEL staining kit (in situ Cell Death Detection kit) was obtained from Roche Diagnostics, (Indianapolis, IN, USA). Primary antibodies used in this study include: Rabbit anti-Caspase-12, rabbit anti-GRP78 (Abcam Ltd., Hong Kong, China), rabbit anti-p-ERK, rabbit anti-ERK antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-β-actin (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).
Gao Z., Liu G., Hu Z., Shi W., Chen B., Zou P, & Li X. (2018). Grape seed proanthocyanidins protect against streptozotocin-induced diabetic nephropathy by attenuating endoplasmic reticulum stress-induced apoptosis. Molecular Medicine Reports, 18(2), 1447-1454.
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2

Immortalized Hepatocyte Culture and Analysis

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Immortalized human hepatocytes were grown in DMEM Dulbecco's modified Eagle medium containing 10% foetal bovine serum, 1% L‐glutamine (Life Technologies, Gaithersburg, MD), 1% penicillin/streptomycin and 1% MEM non‐essential amino acids (Sigma Chemical Co, St Louis, MO). AST and ALT assay kits were purchased from Cayman Chemical. TUNEL staining kit (In Situ Cell Death Detection Kit) was purchased from Roche. The plasmids, pLL3.7_hsa‐miR‐34a (Plasmid #25791)22 and pLL3.7 (Plasmid #11795),23 were from Addgene. Micro RNA array chips were from Affymetrix Inc (Santa Clara, CA).
Iwagami Y., Zou J., Zhang H., Cao K., Ji C., Kim M, & Huang C. (2018). Alcohol‐mediated miR‐34a modulates hepatocyte growth and apoptosis. Journal of Cellular and Molecular Medicine, 22(8), 3987-3995.
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3

Hepatocyte Culture and Wnt Pathway Modulation

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Immortalized human hepatocytes, Huh7, and Hep3B cells were grown in DMEM Dulbecco's Modified Eagle Medium with 10% fetal bovine serum, L -glutamine (Life Technologies, Gaithersburg, MD), and MEM nonessential amino acids (Sigma Chemical Co, St Louis, MO). Alanine aminotransferase enzymatic activity assay kit was purchased from Cayman Chemical. TUNEL staining kit (In Situ Cell Death Detection Kit) was purchased from Roche. CID 11210285 hydrochloride (2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine hydrochloride, AMBMP, N4-(1,3-benzodioxol-5-ylmethyl)-6-(3-methoxyphenyl)-2,4-pyrimidinediamine hydrochloride, Wnt Agonist) and LiCl were purchased from Sigma. SGK1 inhibitor was purchased from Santa Cruz Biotechnology. The plasmids of Foxo3A (FLAG-Foxo3A WT, #8360) and Foxo3A without DNA binding domain (FLAG-Foxo3A TM, #8361)[23 (link)] were from Addgene.
Huang C.K., Yu T., de la Monte S.M., Wands J.R., Derdak Z, & Kim M. (2015). Restoration of Wnt/β-catenin signaling attenuates alcoholic liver disease progression in a rat model. Journal of hepatology, 63(1), 191-198.
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