Ultra low attachment surface culture plates

Manufactured by Corning

The Ultra-Low Attachment Surface culture plates are designed to promote the growth and expansion of cells in a suspension culture. They feature a specialized surface that minimizes cell attachment, encouraging the formation of spheroids, organoids, or suspension cultures. These plates are suitable for a variety of cell types, including stem cells, tumor cells, and other cell lines.

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Lab products found in correlation

Fv1000, by Olympus (1 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

6‐well ultra‐low attachment Surface Polystyrene culture plates Ultra-low attachment surface tissue culture plates Ultra-low attachment surface 6-well culture plates Ultra-low attachment surface plates Ultra-low attachment culture plates Ultra-low attachment surface Ultra-low attachment plates Low attachment plate Ultra Low plates

2 protocols using ultra low attachment surface culture plates

Differentiation of iPSCs into Multiple Lineages

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iPSCs were dissociated as per passaging and re-suspended with LIF withdrawal medium in Ultra-Low Attachment Surface culture plates (Corning). After culturing for 4 days in suspension, embryoid bodies were transferred to gelatin-coated coverslips placed in 24-well plates. The adherent EBs were cultured with EB medium (DMEM containing 15% KSR, 2 mM Glutamine, 0.1 mM MEM) for other 10 days. Immunostaining was then performed with antibodies for specific lineage markers. Images were captured under a confocal microscope (Olympus FV1000) at 60× magnification.

Ma H., Ow J.R., Tan B.C., Goh Z., Feng B., Loh Y.H., Fedele M., Li H, & Wu Q. (2014). The dosage of Patz1 modulates reprogramming process. Scientific Reports, 4, 7519.

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Sphere Formation from Plated Cells

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Cells were plated at the density of 10,000 cells per well in Ultra-Low Attachment Surface culture plates (Corning, Acton, MA) in serum-free DMEM/F12 medium (Invitrogen) with 20ng/ml epidermal growth factor (Invitrogen), 10ng/ml basic fibroblast growth factor (Sigma-Aldrich, St. Louis, MO), 0.4% bovine serum albumin (Sigma-Aldrich), and 5 mg/ml insulin (Sigma-Aldrich). Sphere formation with 50-100 cells per sphere was established 12 days after plating the cells.

Kim M.J., Kim A.R., Jeong J.Y., Kim K.I., Kim T.H., Lee C., Chung K., Ko Y.H, & An H.J. (2017). Correlation of ALDH1 and Notch3 Expression: Clinical implication in Ovarian Carcinomas. Journal of Cancer, 8(16), 3331-3342.

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