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Ab231283

Manufactured by Abcam
Sourced in United States, China

Ab231283 is a monoclonal antibody that recognizes the human CD4 antigen. The antibody is suitable for use in various applications, including flow cytometry and immunohistochemistry.

Automatically generated - may contain errors

2 protocols using ab231283

1

Antibody Validation for Stem Cell Markers

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The following antibodies were used for western blotting: rabbit monoclonal anti-CD24 (1:500, catalog number ab179821, Abcam, MA, USA), rabbit monoclonal anti-CD44 (1:500, catalog number ab189524, Abcam), rabbit recombinant multiclonal anti-CD133 (1:800, catalog number ab278053, Abcam), mouse monoclonal anti-Nanog (1:1000, catalog number ab173368, Abcam), rabbit monoclonal anti-OCT4 (1:500, catalog number ab200834, Abcam), mouse monoclonal anti-SOX2 (1:1000, catalog number ab79351, Abcam), rabbit polyclonal anti-DLG5 (1:1000, catalog number ab231283, Abcam), rabbit polyclonal anti-PRDM16 (1:1000, catalog number ab106410, Abcam), mouse monoclonal anti-ErbB-2 (1:500, catalog number sc-33684, Santa Cruz Biotechnology, CA, USA), and mouse monoclonal anti-β-actin (1:1000, catalog number A5441, Sigma-Aldrich, MO, USA). Other primary antibodies and secondary antibodies and experimental procedures for immunoblotting are provided in the supplementary experimental procedures10 (link).
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2

Immunohistochemical Analysis of DLG5 and PRDM16 in Breast Cancer

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Four-micrometer sections from breast cancer paraffin-embedded tissue samples were used to perform IHC staining by PV-6000 detection kits, and ZLI-9032 DAB substrate kit (Beijing Zhongshan golden bridge Biotechnology Co. Ltd., Beijing, China) The sections were incubated in anti-DLG5 antibody (1:300, catalog number ab231283, Abcam) or anti-PRDM16 antibody (1:400, catalog number ab106410, Abcam) at 4 °C overnight. The results of IHC staining were evaluated by an experienced pathologist, and quantification of the reaction was performed using the histoscore system as previously described48 (link).
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