For chemotaxis assays, isolated KLS cells were suspended in migration medium comprised of RPMI-1640 medium, supplemented with 0.5% BSA, and then seeded in 8 µm pore transwell plates (Corning-Costar Corp). Murine SDF-1α (50, 100, or 200ng/mL; Sigma-Aldrich) or S1P (10 or 100nM; Sigma-Aldrich) was added to the lower chamber (specific concentration is indicated for each figure). KLS cells (1×104 or 5×104 cells) were placed in the upper well and incubated at 37°C for 4 hours and the number of cells in the lower wells was determined by flow cytometry. The migration index was calculated as the fold of increase in migration to the chemoattractant relative to baseline migration (without chemoattractant). Levels of SDF-1α and S1P were selected based on previous reports32 (link),33 (link).
8 m pore transwell plates
Manufactured by Corning
The 8 μm pore transwell plates are a laboratory equipment product designed for cell culture applications. These plates feature a membrane with 8 μm pores that allow for the selective passage of cells and molecules between the upper and lower chambers. The plates provide a versatile platform for various in vitro experiments and assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 8 m pore transwell plates
1
Chemotaxis Assay for KLS Cells
2
Chemotaxis Assay for KLS Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For chemotaxis assays, isolated KLS cells were suspended in migration medium comprised of RPMI-1640 medium, supplemented with 0.5% BSA, and then seeded in 8 µm pore transwell plates (Corning-Costar Corp). Murine SDF-1α (50, 100, or 200ng/mL; Sigma-Aldrich) or S1P (10 or 100nM; Sigma-Aldrich) was added to the lower chamber (specific concentration is indicated for each figure). KLS cells (1×104 or 5×104 cells) were placed in the upper well and incubated at 37°C for 4 hours and the number of cells in the lower wells was determined by flow cytometry. The migration index was calculated as the fold of increase in migration to the chemoattractant relative to baseline migration (without chemoattractant). Levels of SDF-1α and S1P were selected based on previous reports32 (link),33 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!