The Transwell chambers with 8 μm pore size filters (Corning Incorporated, Corning, NY, USA) were coated with Matrigel (BD Biosciences, San Jose, CA, USA). Cells that were treated with or without juglone (12.5, 25, 50, or 100 μM) for 24 hr were suspended in 0.1 % FBS. They were seeded in the upper chamber in 200 μl IMDM medium, and 600 μl IMDM medium containing 10 % FBS was added to the bottom chamber. Following incubation, the remaining cells in the upper chamber were gently removed with a cotton swab. Cells that migrated to the lower side of the inserts were stained with 0.1 % crystal violet and observed under light microscopy (Olympus, Tokyo, Japan). Then, crystal violet was completely dissolved in 33% acetic acid. The absorbance was detected at 570 nm by microplate reader (Bio-Rad, USA).
Transwell chambers with 8 μm pore size filters
Manufactured by Corning
Sourced in United States
Transwell chambers with 8 μm pore size filters are laboratory equipment used for cell culture studies. These chambers feature a porous membrane that allows for the exchange of nutrients, gases, and signaling molecules between the upper and lower compartments.
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Lab products found in correlation
2 protocols using transwell chambers with 8 μm pore size filters
1
Juglone Effects on Cell Migration
2
Cell Migration Assay with Transwell
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Transwell chambers with 8 μm pore-size filters (Corning Inc., Corning, NY, USA) were used for cell migration assay. After IRS1 knockdown, KKU-213A (2 × 104 cells) and KKU-213B (5 × 104 cells) in serum-free medium were added to the upper chambers, while the lower chambers were filled with complete medium containing 10% fetal bovine serum. After 18 h of incubation, cells were allowed to migrate through the underside of the porous filters. The migrating cells were fixed with absolute methanol and stained with Mayer’s hematoxylin. The stained cells were counted under a microscope.
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