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Fitc anti mouse cd3ε antibody

Manufactured by BioLegend
Sourced in United States

The FITC anti-mouse CD3ε antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD3ε chain of the T cell receptor complex in mice. It can be used to identify and quantify T cells in flow cytometry applications.

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4 protocols using fitc anti mouse cd3ε antibody

1

T Cell Phenotyping Post Vaccination

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T cell population detection post vaccination was assessed using flow cytometry assay and 50 μL whole EDTA blood. Red blood cells were lysed with ACK buffer and washed twice with 1% (v/v) FBS in 1× PBS. The fluorophore-conjugated antibody mixture was added to the sample and incubated on ice for 20 min. FITC anti-mouse CD3ε Antibody (Cat#10036), APC/Cyanine7 anti-mouse CD8a Antibody (Cat#100714), APC anti-mouse CD4 Antibody (Cat#100412), and PE anti-mouse CD19 Antibody (Cat#115508) were bought from Biolegend (San Diego, CA, USA). Cells were then resuspended with 1% FBS in 1× PBS after washing 3 times. All samples were run on the flow cytometer.
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2

Edible Bird Nest Powder Immunomodulatory Effects

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A raw EBN sample (rEBN) was purchased from Surat-Thani, Thailand. Aiko Edible Bird Nest Pattani Part., Ltd. (Thailand) provided the processed EBN powder (pEBN). Thermo Fisher Scientific K.K. (Japan) supplied the RPMI 1640 medium and Dulbecco's Modified Eagle Medium (DMEM). FITC anti-mouse MHC-II, FITC anti-mouse CD80, and FITC anti-mouse CD86 were purchased from eBioscience, Inc. (USA). FITC anti-mouse CD3ε antibody, PE anti-mouse CD19 antibody, anti-mouse CD16/32 antibody, and recombinant mouse IL-2 were purchased from BioLegend, Inc. (U.S.A.). Falcon® 70 μm Cell Strainer was purchased from Corning, Inc. (USA). Fetal bovine serum (FBS), l-Glutamine, Penicillin-Streptomycin, and β-mercaptoethanol were purchased from Nacalai Tesque, Inc. (Japan). The filter paper was purchased from ADVANTEC Co., Ltd., Japan). THUNDERBIRD™ - Probe and SYBR® were purchased from Toyobo Co., Ltd., JAPAN.
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3

Comprehensive Immune Cell Profiling

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AT-infiltrating stromal cells were stained with FITC anti-mouse CD3ε antibody, PE anti-mouse CD4 antibody, PE/Cy7 anti-mouse CD25 antibody, APC anti-mouse CD8a antibody, FITC anti-mouse CD127 antibody, PerCP/Cy5.5 anti-mouse CD4 antibody, APC anti-mouse CD25 antibody (these antibodies were purchased from Biolegend, San Diego, CA, USA), PE anti-mouse/Foxp3 antibody (Invitrogen, Carlsbad, CA, USA), PE anti-mouse CD11b antibody (Biolegend), PE/Cy7 anti-mouse Ly-6C antibody (Biolegend, San Diego, CA, USA) and APC anti-toll-like receptor 4 (TLR4) antibody (Invitrogen, Waltham, MA, USA), and analyzed using an Accuri C6 (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
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4

Splenic Lymphocyte CD4+/CD8+ Subclass Analysis

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Splenic lymphocyte CD4+/CD8+ subclass analysis was performed using flow cytometry. An amount of 97 µL of cell staining buffer (BioLegend, San Diego, CA, USA, Cat. No#420201) and 0.5 µL of TruStain FcXTM PLUS (anti-mouse CD16/32) (BioLegend, Cat. No#156604) antibody were transferred to 1.5 mL Eppendorf (EP) tubes containing 1.5 × 106 cells, mixed softly, and then used to stain the cells for 10 min at RT in the dark. Thereafter, 0.5 µL of FITC anti-mouse CD3ε antibody (BioLegend, San Diego, CA, USA, Cat. No#100204), 1.25 µL of PerCP/Cyanine5.5 anti-mouse CD8α antibody (BioLegend, San Diego, CA, USA, Cat. No#100734), and 1.25 µL of PE anti-mouse CD4 antibody (BioLegend, San Diego, CA, USA, Cat. No#100512) were added to the EP tubes, mixed softly, and then used to stain the cells for 30 min at 4 °C in the dark. Then, the EP tubes were centrifuged at 200× g for 5 min, and the supernatant was discarded. Next, 1 mL of PBS was added to make a cell suspension, centrifuged at 4 °C, 200× g for 5 min, and the supernatant was then removed. Cells were centrifuged twice, and 250 µL of cell staining buffer was added for cell suspension. Finally, the cells were analyzed by FACSVerse flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA), and all dates were analyzed using FlowJoV_10 software.
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