Human bdnf duoset elisa

Manufactured by R&D Systems

Sourced in Canada

The Human BDNF DuoSet ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of human brain-derived neurotrophic factor (BDNF) levels in cell culture supernatants, serum, and plasma samples. It is designed to detect and quantify BDNF protein concentrations.

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Lab products found in correlation

Fluostar omega microplate reader, by BMG Labtech (1 mentions) Safire plate reader, by Tecan (1 mentions)

Close spelling variants of this product

Human/Mouse BDNF DuoSet ELISA Human BDNF Duoset ELISA Kit Human Pro-BDNF DuoSet ELISA kit Human/Mouse BDNF DuoSet ELISA kit Human ELISA DuoSet BDNF DuoSets DuoSet ELISA DuoSets ELISAs

2 protocols using human bdnf duoset elisa

Quantifying BDNF Levels in Serum

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The BDNF analysis was conducted using a commercially available ELISA kit (Human BDNF DuoSet ELISA, R&D Systems) according to the manufacturer's protocol. Plates were blocked for 2 hours in reagent diluents (1% BSA/PBS). Serum samples for BDNF were run undiluted. Plates were incubated with 100 μl of serum samples overnight at 4°C. All samples and standards were run in duplicates. The optical density was measured using a FLUOstar Omega microplate reader (BMG Labtech, Ortenberg, Germany) at 450 nm excitation and 570 nm emission wavelengths. BDNF concentration was calculated from the standard curve generated from human recombinant BDNF provided by the manufacturer. The mean values of measurements were analyzed by a nonlinear regression fit algorithm using GraphPad Prism version 4.0 (GraphPad Software, San Diego, CA).

Banasiak‐Cieślar H., Wiener D., Kuszczyk M., Dobrzyńska K, & Polanowski A. (2023). Proline‐rich polypeptides (Colostrinin®/COLOCO ®) modulate BDNF concentration in blood affecting cognitive function in adults: A double‐blind randomized placebo‐controlled study. Food Science & Nutrition, 11(3), 1477-1485.

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Quantifying NGF and BDNF Levels by ELISA

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NGF-immunoreactive protein levels were measured by two-site, sandwich ELISA using an affinity-purified rabbit polyclonal antibody MC-51, following protocols from Boehringer Mannheim, with fluorescent substrate 4-methylumbelliferyl β -D-galactoside. Fluorescence was read on a Safire plate reader (Tecan, Männedorf, Switzerland) with background subtracted. Samples were measured in duplicate on separate plates.
BDNF levels in ventricular CSF were measured in duplicate using the human BDNF DuoSet ELISA (R&D Systems, Minneapolis, MN) according to the manufacturer’s protocols, but using Thermo Scientific QuantaBlu Fluorogenic Peroxidase substrate (Life Technologies, Burlington, Canada) in place of the colorimetric substrate tetramethylbenzidine-H₂O₂ to increase sensitivity. Three samples were removed from analysis based on high total protein levels (>3.5 mg/ml), and one outlier in the MCI group was removed because BDNF levels were more than 2 standard deviations higher than the mean.

Counts S.E., He B., Prout J.G., Michalski B., Farotti L., Fahnestock M, & Mufson E.J. (2016). Cerebrospinal Fluid proNGF: A Putative Biomarker for Early Alzheimer’s Disease. Current Alzheimer research, 13(7), 800-808.

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