Lc ms grade acetonitrile

LC-MS grade acetonitrile, methanol, ammonium acetate, and formic acid were purchased from CNW Technologies (Duesseldorf, Germany). 2-Chloro-L-phenylalanine was purchased from Shanghai HB Biotech Co., Ltd. (Shanghai, China). Deionized water was prepared on a Millipore Milli-Q water purification system (Billerica, MA, USA). Apigenin was obtained from China National Engineering Research Centre for Solid Preparation Manufacturing Technology (Beijing, China). Psoralen was purchased from China Food and Drug Control Institute (Beijing, China). Assay kits for aminotransferase (ALT) and aspartate aminotransferase (AST) were purchased from Nanjing Jiancheng Biological Engineering (Nanjing, China). RFH was purchased from Baining Pharmaceutical Co., Ltd. (Guangzhou, Guangdong).

Raw SO (the dried aerial part of Siegesbeckia orientalis L.) was collected from Ganzhou (Jiangxi province, China). Rice wine was purchased from Zhejiang Tapai Shaoxing Wine Co., Ltd. (Zhejiang province, China). LC–MS grade acetonitrile, methanol, and formic acid (FA) were purchased from CNW Technologies (Germany). Kirenol was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Adonitol was purchased from Sigma-Aldrich (MO, United States). Milli-Q water was prepared using a Milli-Q system (Millipore, MA, United States). The commercial kits including creatine kinase (CK), lactate dehydrogenase (LDH), urea, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were purchased from Shenzhen Mindray Bio-Medical Electronics Co., Ltd. (Guangdong province, China). Malondialdehyde (MDA) was purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu province, China).