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Peg nh2

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PEG-NH2 is a polyethylene glycol (PEG) derivative with an amino (NH2) functional group. It is a water-soluble, non-toxic, and biocompatible compound commonly used in various laboratory applications.

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Lab products found in correlation

500mhz system, by Agilent Technologies (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Ru dpp 3 cl2, by Merck Group (2 mentions) Rpmi medium, by Thermo Fisher Scientific (2 mentions) Dmem medium, by Thermo Fisher Scientific (2 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (2 mentions) Cy7 peg nh2, by Nanocs (2 mentions) Trypsin edta, by Nanocs (2 mentions) 9 10 anthracenedipropanoic acid, by Merck Group (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Dihydrorhodamine 123 dhr123, by Merck Group (1 mentions) N methylpyrrolidone, by Merck Group (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Methylene blue, by Merck Group (1 mentions) Anti h2ax ps139, by BD (1 mentions) Bismuth nitrate pentahydrate, by Merck Group (1 mentions) 2 7 dichlorodihydrofluorescein diacetate, by Merck Group (1 mentions) Alexa fluor 647 mouse anti h2ax ps139, by BD (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Dulbecco s minimum essential medium, by Thermo Fisher Scientific (1 mentions)

7 protocols using peg nh2

1

Synthesis of PEGylated Nanomaterials

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Bulk BP was purchased from Macklin Company (Shanghai, China). PEG-NH2 (5000 Da) was purchased from Nanocs, Inc. (New York, USA). Other reagents were analytical grade. Ultrapure water (25 °C, 18.25 MΩ cm) was used for water-based dispersions.
Xie Z., Peng M., Lu R., Meng X., Liang W., Li Z., Qiu M., Zhang B., Nie G., Xie N., Zhang H, & Prasad P.N. (2020). Black phosphorus-based photothermal therapy with aCD47-mediated immune checkpoint blockade for enhanced cancer immunotherapy. Light, Science & Applications, 9, 161.
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2

PEGylation of Bilirubin for Therapeutic Use

Cited 1 time
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PEG-BR was prepared as previously described with modifications48 (link). 75 μmol of bilirubin and 33.75 μmol of EDC were added to 0.6 ml of DMSO containing 225 μl of TEA and 52 μmol of NHS for 10 min at RT. After then, 15 μmol of polyethylene glycol 20K-amine (PEG-NH2, Nanocs) and the mixture was mixed for 4 h under nitrogen gas. Subsequently, the mixture was added to 50 ml of chloroform and then the organic solvents were washed by 50 ml of 0.1M HCl twice, followed by washing with 50 ml of 0.1M NaHCO3 twice and finally washed with 50 ml of distilled water twice. The organic layer was evaporated, and 50 ml of methanol was added to the residue. After centrifugation at 3,000 x g for 10 min, the supernatant was collected and then evaporated. Dialysis was performed as described in the previous section. To yield PEGylated bilirubin (PEG-BR), lyophilization was performed. The final structure was confirmed by 1H-NMR. 1H-NMR spectra were recorded on a Varian 500MHz system; chemical shifts represent ppm downfield from tetramethylsilane.
Lee Y., Sugihara K., Gillilland MG I.I.I., Jon S., Kamada N, & Moon J.J. (2019). Hyaluronic acid-bilirubin nanomedicine for targeted modulation of dysregulated intestinal barrier, microbiome, and immune responses in colitis.. Nature materials, 19(1), 118-126.
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3

Vermiculite-Based Nanoparticle Synthesis

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Vermiculite was supplied from Yunnan province, China. Cy7-PEG-NH2 (MW: 5k) and PEG-NH2 (MW: 5k) were purchased from Nanocs Inc. PBS (pH 7.4 and 5.5), DMEM medium, RPMI medium, trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Gibco Life Technologies. 9,10-anthracenedipropanoic acid (ABDA), [Ru(dpp)3]Cl2 (RDPP), H2O2 (30%), N-methyl-pyrrolidone (NMP), methylene blue (MB), 3,3′,5,5′-tetramethylbenzidine (TMB), glutathione, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), and dihydrorhodamine 123 (DHR123) were supplied by Sigma-Aldrich.
Ji X., Ge L., Liu C., Tang Z., Xiao Y., Chen W., Lei Z., Gao W., Blake S., De D., Shi B., Zeng X., Kong N., Zhang X, & Tao W. (2021). Capturing functional two-dimensional nanosheets from sandwich-structure vermiculite for cancer theranostics. Nature Communications, 12, 1124.
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4

PEGylation of Bilirubin for Therapeutic Use

Cited 1 time
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PEG-BR was prepared as previously described with modifications48 (link). 75 μmol of bilirubin and 33.75 μmol of EDC were added to 0.6 ml of DMSO containing 225 μl of TEA and 52 μmol of NHS for 10 min at RT. After then, 15 μmol of polyethylene glycol 20K-amine (PEG-NH2, Nanocs) and the mixture was mixed for 4 h under nitrogen gas. Subsequently, the mixture was added to 50 ml of chloroform and then the organic solvents were washed by 50 ml of 0.1M HCl twice, followed by washing with 50 ml of 0.1M NaHCO3 twice and finally washed with 50 ml of distilled water twice. The organic layer was evaporated, and 50 ml of methanol was added to the residue. After centrifugation at 3,000 x g for 10 min, the supernatant was collected and then evaporated. Dialysis was performed as described in the previous section. To yield PEGylated bilirubin (PEG-BR), lyophilization was performed. The final structure was confirmed by 1H-NMR. 1H-NMR spectra were recorded on a Varian 500MHz system; chemical shifts represent ppm downfield from tetramethylsilane.
Lee Y., Sugihara K., Gillilland MG I.I.I., Jon S., Kamada N, & Moon J.J. (2019). Hyaluronic acid-bilirubin nanomedicine for targeted modulation of dysregulated intestinal barrier, microbiome, and immune responses in colitis.. Nature materials, 19(1), 118-126.
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5

Oxidative Stress Assay Protocol

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Ferric chloride (FeCl3), sodiun hydroxide (NaOH), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), methylene blue (MB), [Ru(dpp)3]Cl2 (RDPP), glutathione, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), and H2O2 (30%) were supplied by Sigma-Aldrich. PEG-NH2 (MW: 5k) and Cy7-PEG-NH2 (MW: 5k) were supplied by Nanocs Inc. Trypsin-EDTA, phosphate buffer saline (PBS, pH 7.4), fetal bovine serum (FBS), RPMI medium, and DMEM medium were purchased from Gibco Life Technologies. 8-OhdG DNA Damage Quantification Direct Kit was purchased from EpiQuik™ Colorimetric. C-CAS3 (Asp175) and Anti-H2AX (pS139) antibodies were purchased from BD Pharmingen™.
Kang Y., Mao Z., Wang Y., Pan C., Ou M., Zhang H., Zeng W, & Ji X. (2022). Design of a two-dimensional interplanar heterojunction for catalytic cancer therapy. Nature Communications, 13, 2425.
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6

Nanoparticle-based Apoptosis Detection

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Bismuth nitrate pentahydrate [Bi(NO3)3·5H2O], sodium chloride (NaCl), potassium borohydride (KBH4), hydrogen dioxide (H2O2), 2,7-dichlorodihydrofluorescein diacetate, MB, GSH, [Ru(dpp)3]Cl2, and DTNB were obtained from Sigma-Aldrich. PEG-NH2 [molecular weight (MW), 5000] and Cy5.5-PEG-NH2 (MW, 5000) were provided by Nanocs Inc. Trypsin-EDTA, Dulbecco’s minimum essential medium, RPMI 1640 medium, fetal bovine serum, and PBS (pH 7.4) were provided by Gibco Life Technologies. Alexa Fluor 647 mouse anti-H2AX (pS139) and anti-cleaved poly(adenosine 5′-diphosphate–ribose) polymerase (Asp214) antibodies were secured from BD Pharmingen. Normal human liver cells (HL-7702; catalog no. 77402), human embryonic kidney cells (HEK293, catalog no. CRL-1573), normal human mammary epithelial cells (MCF-10A, catalog no. CRL-10781), human breast cancer cell (MCF-7, catalog no. HTB-22), and human hepatoma cells (HepG2, catalog no. HB-8065) were supplied by the American Type Culture Collection (ATCC). ATCC used morphology, karyotyping, and polymerase chain reaction–based approaches to confirm the identity of human cell lines and rule out intra- and interspecies contamination. Also, the cell line was frequently evaluated through its morphological features.
Chen L., Mao Z., Wang Y., Kang Y., Wang Y., Mei L, & Ji X. (2022). Edge modification facilitated heterogenization and exfoliation of two-dimensional nanomaterials for cancer catalytic therapy. Science Advances, 8(39), eabo7372.
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7

Synthesis of PEGylated Bilirubin

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75 µmol of bilirubin and 33.75 µmol of EDC were added to 0.6 ml of DMSO containing 225 µl of TEA and 52 µmol of NHS for 10 min at RT. After then, 15 µmol of polyethylene glycol 20K-amine (PEG-NH2, Nanocs) was added and mixed for 4 h under nitrogen gas. Subsequently, the mixture was added to 50 ml of chloroform, and the organic solvent was washed with 50 ml of 0.1 M HCl twice, followed by washing with 50 ml of 0.1 M NaHCO3 twice and washing with 50 ml of distilled water twice. The organic layer was evaporated, and 50 ml of methanol was added to the residue. After centrifugation at 3000 × g for 10 min, the supernatant was collected and then evaporated. Dialysis was performed as described in the previous section. To yield PEGylated bilirubin (PEG-BR), lyophilization was performed. The final structure was confirmed by 1H-NMR. 1H-NMR spectra were recorded on a Varian 500 MHz system; chemical shifts represent ppm downfield from tetramethylsilane.
Lee Y., Shinn J., Xu C., Dobson H.E., Neamati N, & Moon J.J. (2023). Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy. Nature Communications, 14, 4771.
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