Poly 4 styrene sulfonate sodium salt

Poly(allylamine hydrochloride) (PAH, Mw = 17.5 kDa), poly(4-styrene sulfonate) sodium salt (PSS, Mw = 70 kDa), calcium chloride dihydrate, sodium carbonate, sodium chloride, ethylenediaminetetraacetic acid (EDTA), dextran sulfate sodium salt (DS, Mw = 40 kDa), rhodamine B, rhodamine 6G, pyronin B, fluorescein, acridine yellow, acridine orange, thiazine red, and polyvinyl alcohol were purchased from Sigma.
Dulbecco's modified Eagle's medium (DMEM), Dulbecco's modified Eagle's medium F12 (DMEM F12), phosphate-buffered saline (PBS), fetal bovine serum (FBS), trypsin EDTA, bovine serum albumin (BSA), calcein-AM fluorescent dye, hoechst 33258 dye, dimethyl sulfoxide (DMSO) and MTT cell proliferation assay ((3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay) kit were purchased from Thermo Fisher Scientific.
Deionized (DI) water (resistivity 18.2 MΩ cm at 25 °C) from the Direct-Q water purification system was used to prepare all solutions.

Poly(allylamine hydrochloride) (PAH, M_W 58,000), poly(4-styrenesulfonate) sodium salt (PSS, M_W = 70,000), poly(acrylic acid) (PAA, M_W 100,000), poly(diallyldimethylammonium chloride) (PDDA, M_W 200,000–350,000), poly(ethylenimine) (PEI, M_W ~750,000) were all purchased from Sigma (St. Louis, USA). Poly(dimethylsiloxane) (PDMS) kit (Sylgard 184) was purchased from Dow-Corning, Midland, USA. In addition PDMS (Elastosil, RT 602) from Wacker AG, München, Germany was used. Deionized (DI) water from a Milli-Q (Millipore) water purification system was used to make all solutions. All the chemicals were used as received without further purification.

Poly(4-styrene sulfonate) sodium salt (PSS, Mw = 70 kDa), poly(allylamine hydrochloride) (PAH, Mw = 17.5 kDa), dextran sulfate sodium salt (DS, Mw = 40 kDa), ethylenediaminetetraacetic acid disodium salt, calcium chloride, sodium carbonate, and Rhodamine B were purchased from Sigma-Aldrich (Taufkirchen, Germany). Dimethyl sulfoxide was purchased from Merck (Darmstadt, Germany). Cyanine 7 NHS ester (Cy7) was obtained from Lumiprobe (Moscow, Russia). Polyvinyl alcohol (PVA) powder (Mw = 72,000 g/mol with a degree of hydrolysis of 85–89%) was supplied by AppliChem GmbH (Darmstadt, Germany). All the chemicals were used without further purification. Deionized water produced using a Milli-Q Plus 185 water treatment system (Merck Millipore, Darmstadt, Germany) was used to prepare all solutions. Phosphate-buffered saline (PBS), Calcein AM fluorescent dye, and the MTT cell proliferation assay kit were purchased from Thermo Fisher Scientific (Eugene, OR, USA).