Mouse anti human monoclonal β catenin

TSA injection (sulfotanshinone sodium injection, 5 mg/mL; First Biochemical Pharmaceutical Co. Ltd., Shanghai, China) was used in the in vivo experiments. TSA monomer (Sigma, St. Louis, MO), a lyophilized powder (99.99% purity), which was first dissolved in dimethyl sulfoxide, and then diluted with phosphate-buffered saline (PBS) to the required concentration, was used in the in vitro experiments. Antibodies used for immunoblotting and/or immunohistochemistry were as follows: mouse anti-human monoclonal β-catenin (Abcam, Cambridge, MA, USA), rabbit anti-human polyclonal glycogen synthase kinase-3 beta (GSK-3β; Epitomics, Burlingame, CA, USA), rabbit anti-human polyclonal SOX2 (Epitomics, Burlingame, CA, USA), mouse anti-human monoclonal OCT4 (Abcam, Cambridge, MA, USA), mouse anti-human monoclonal Nanog (Abcam, Cambridge, MA, USA), rabbit anti-human polyclonal VEGF (Abcam, Cambridge, MA, USA), mouse anti-human monoclonal CD34 (Abcam, Cambridge, MA, USA), and mouse anti-human monoclonal actin (Beyotime, Shanghai, China).

Oxaliplatin and the IGF1R inhibitor PQ401 were purchased from Sigma Chemical Company (St Louis, MO, USA). For antibodies used in immunoblotting and/or immunochemistry were purchased as follows: mouse anti-human monoclonal cluster of differentiation (CD)44 (Abcam, Cambridge, MA, USA), used at concentration of 5 μg/ml; rabbit anti-human monoclonal CD90 (Abcam), used at concentration of 1 μg/ml; mouse anti-human monoclonal epithelial cell adhesion molecule (EpCAM) (Abcam), used at concentration of 5 μg/ml; mouse anti-human monoclonal aldehyde dehydrogenase (ALDH) (Abgent, San Diego, CA, USA), used at concentration of 3 μg/ml; mouse anti-human monoclonal β-catenin (Abcam), used at concentration of 2 μg/ml; mouse anti-human monoclonal IGF1 (Abcam), used at concentration of 1 μg/ml; rabbit anti-human monoclonal (sex determining region Y)-box 2 [Homo sapiens (human)] (SOX2) (Abcam), used at 1∶500 dilution; mouse anti-human monoclonal octamer-binding transcription factor 4 (OCT4) (Abcam), used at 1∶100 dilution; mouse anti-human monoclonal E-cadherin (Abcam), used at 1∶100 dilution; mouse anti-human monoclonal vimentin (Abcam), used at concentration of 3 μg/ml; and rabbit anti-human monoclonal β-actin (Epitomics, Burlingame, CA, USA), used at 1∶1000 dilution.