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Sodium carbonate

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Sodium carbonate is an inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used in various industrial and laboratory applications.

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15 protocols using sodium carbonate

1

Extraction and Characterization of Bioactive Compounds

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SR, SPR, and ZPR were provided by Guizhou Zhongyi Food Co., Ltd.(Guiyang, China), Jialijia Agricultural Development Co., Ltd., Sinan County, Tongren City, Guizhou Province, China, and Qin Likang Team of Guizhou University, respectively. GL (Ganoderma destructans CBS 139793) was provided by Min Yuan Fungi (Chongqing, China). Bromocresol green, sodium carbonate, potassium bromide, and so forth were purchased from McLean Biochemical Technology Co., Ltd (Shanghai, China). Starch kit, potassium dichromate, and phloroglucinol were purchased from Beijing Solarbio Technology Co., Ltd. Tianjin Kemio Chemical Reagent Co., Ltd. provided gold chloride, aluminum trichloride, and glucose, as well as other analytical-grade chemical reagents which were not previously mentioned.
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2

Styrene Polymerization and Bentonite Fluid

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Styrene (St, 99%) and ammonium persulfate ((NH4)2S2O8, AR) were purchased from Sinopharm Reagent Co. Sodium p-Styrene sulfonate (SSS, 90%) and sodium carbonate (99.8%) were provided by Shanghai Maclean Biochemical Technology Co., Shanghai, China. Polyethylene glycol octylphenyl ether (OP-10, 99%) was obtained from Beijing J&K Science Co., Beijing, China. Bentonite is an industrial product from Huaian County Tengfei Bentonite Development Company, Ltd., Zhangjiakou, China. The based bentonite fluid (DF) was made by adding 4 wt% bentonite and 0.3 wt% sodium carbonate to water and stirring for 24 h [2 (link),8 (link),9 (link)].
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3

Buckwheat Seed Antioxidant Characterization

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Tartary buckwheat seeds (Hefeng No. 1) were cultivated in Datong, Shanxi Province, China, and were acquired from Beijing Green Valley Sprout Co., Ltd. (Beijing, China). Rutin, gallic acid, and γ-Amino butyric acid standard were purchased from Shanghai solarbio Bioscience & Technology Co., Ltd. (Shanghai, China). The analytical grade chemicals, such as Folin-Ciocalteu reagent, gallic acid (GA), sodium carbonate, sodium hypochlorite, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid, glucose anhydrous, aluminum chloride, potassium acetate, o-phthalaldehyde, copper sulfate, potassium sulfate, sulfuric acid, boric acid, ethanol, ethyl acetate, hydrochloric acid, trichloroacetic acid, sodium acetate, phenol, potassium persulfate, sodium hydroxide, and aluminum chloride were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). In addition, 2,2-diphenyl picryl hydrazyl (DPPH), acetone, and methanol (HPLC grade) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Total antioxidant capacity (T-AOC) kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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4

3T3-L1 Preadipocyte Assay Protocol

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3T3-L1 mouse preadipocytes were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). High glucose DMEM, low glucose DMEM, Pen-Strep solution (P/S), insulin, certified fetal bovine serum (FBS), special newborn calf serum (NBCS), and phosphate buffered saline (PBS) were purchased from Biological Industries (Shanghai, China). The glucose test kit was purchased from Rongsheng Biotech Co., Ltd. (Shanghai, China). α-Glucosidase (solid), 3,5-dinitrosalicylic acid, p-nitrophenyl α-D-glucopyranoside (PNPG), and ascorbic acid were purchased from Yuanye Biotech Co., Ltd. (Shanghai, China). Acarbose and rutin were obtained from Solarbio (Beijing, China). CellTiter 96® AQueous One Solution Reagent (Promega Corporation, Madison, WI, USA). DPPH, ABTS, and FRAP detection reagents were purchased from Suzhou Comin Biotechnology Co., Ltd. (Jiangsu, China). Sodium nitrite, aluminum nitrate, sodium carbonate, and sodium hydroxide were purchased from MACKLIN (Shanghai, China).
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5

Silk Fibroin-based Hydrogel Synthesis

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The cocoon shell of Bombyx Mori silkworm was purchased from Chihe town, Shaanxi, China. Gelatin type A (300 bloom from porcine skin) was purchased from Merck Co., Ltd. Sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O) and sodium hydroxide (NaOH) were purchased from Sinopharm Co., Ltd. Calcium chloride anhydrous (CaCl2) and sodium carbonate (Na2CO3) were obtained from Macklin Co., Ltd. Methacrylic anhydride (MA) was purchased from Sigma-Aldrich Co., Ltd. Irgacure 2959 was obtained from BASF Co., Ltd. All chemicals and solvents purchased were used as received without further purification.
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6

Antioxidant Evaluation of S. involucratus

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Folin's reaction solution, sodium carbonate, 2,4,6-tripyridyltriazine (TPTZ), 2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium persulfate, quercetin, trolox, gallic acid, FeCl3, sodium acetate, and AlCl3 were from Macklin Biochemical Co., Ltd. (Shanghai, China). Methanol and acetonitrile were purchased from Thermo Fisher Scientific (MA, USA), and formic acid was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Distilled water was obtained from a distilled water machine (EPED-E1-10TJ).
S. involucratus (King ex Bak.) Craib was collected from Yangshan County, Qingyuan City, Guangdong Province, China.
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7

Synthesis of Bismuth Vanadate Compounds

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Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, 99%), potassium iodide (KI, 99%), and p-benzoquinone (C6H4O2, 99%) for the preparation of BVO in the experiment were from Aladdin (Shanghai, China). Vanadium acetylacetonate (C10H14O5V, 99%) and vanadium pentoxide (V2O5, 99%) were from MACKLIN (Shanghai, China). The solvent used was nitric acid (HNO3) from MACKLIN. Dimethyl sulfoxide (C2H6OS) was from Aladdin. Acetonitrile (CH3CN) was obtained from Aladdin, sodium bicarbonate (NaHCO3, ≥99.8%) and sodium carbonate (Na2CO3, ≥99.8%) were obtained from MACKLIN. Potassium hydroxide (KOH, 90%), sodium hydroxide, sodium sulfite, and boric acid (H3BO3, 99.99%) come from MACKLIN. Benzyl alcohol (C6H5CH2OH, >99.0%) and benzaldehyde (C7H6O, >99.0%) are sourced from Aladdin.
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8

Synthesis of Seawater-Soluble Polymers

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Acrylamide (AM, 99%), acrylic(AA, 98%), N-vinyl pyrrolidone (NVP, 98%), 2-Acrylamide-2-methylpropanesulfonic acid (AMPS, industrial grade), pentaerythritol triallyl ether (PTE, 99%), cetyl dimethylallyl ammonium chloride (C16DMAAC, 80%), potassium formate (HCOOK, industrial grade), sodium hydroxide (NaOH, AR), sodium carbonate (Na2CO3, AR), potassium persulfate (KPS, AR), sodium bisulfite (NaHSO3, AR), all the above materials were purchased from Shanghai Macklin Biochemical Co. LTD, China and were used without any further purification. Seawater is prepared by artificial seawater as Table 1.

Artificial seawater formulation.

SubstancesConcentration (g/L)
NaCl21.86 ± 0.01
Na2SO43.23 ± 0.01
MgCl24.53 ± 0.01
CaCl20.93 ± 0.01
KCl0.64 ± 0.01
NaHCO30.17 ± 0.01
Na2CO30.02 ± 0.01
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9

Electrochemical Sensing of Biomolecules

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Phosphate buffer saline (PBS, 0.1 M, pH 7.0) was purchased from Source Leaf Biotechnology (Shanghai, China). Potassium ferricyanide (K3Fe(CN)6, ≥99.5%), isopropyl alcohol (≥99.7%) and potassium chloride (KCl, 99.5%) were provided by Aladdin (Shanghai, China). Dopamine hydrochloride (DA) and uric acid (UA, ≥99%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). l-Ascorbic acid (AA, >99%) citric acid (CA, 99.5%), l-cysteine (l-Cys, 99%), sodium carbonate (Na2CO3, 99.5%), calcium chloride (CaCl2, 99.99%), and magnesium sulfate (MgSO4, 99.5%) were bought from Macklin (Shanghai, China). Ethanol, zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), cobalt nitrate hydrate (Co(NO3)2·6H2O, 99%), dibasic sodium phosphate (Na2HPO4) and 2-methylimidazole (C4H6N2, 98%) were obtained from Adamas (Shanghai, China). All of the chemicals were used without further purification. The water used in the experiments was obtained from a Milli-Q system (18.2 MΩ cm−1).
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10

Hemicellulose-based Electrolyte Additives

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The hemicelluloses extracted from the dissolving pulp were supplied by a viscose fiber mill in Xinjiang, China. The preparation of the hemicelluloses can be found in our previous literature [30 (link)]. Potassium carbonate (K2CO3, 99%), potassium hydroxide (KOH, 95%), sodium carbonate (Na2CO3, 99.5%), Zinc chloride (ZnCl2, 98%), Lithium sulfate (Li2SO4, 99%), polyvinylidene fluoride (PVDF), N-methyl pyrrolidone (NMP, 99%) were purchased from Shanghai Macklin Biochemical Co., Ltd., Shanghai, China. Acetylene black was obtained from Tianjin Ebory Chemical Co., Ltd., Tianjin, China. Hydrochloric acid (HCl, 37 wt%) and sulfuric acid (H2SO4, 95 wt%) were purchased from Shanghai Chemical Reagent Co., Ltd., Shanghai, China. HCl, H2SO4, KOH, K2CO3, Na2CO3, ZnCl2, Li2SO4, PVDF, NMP, and acetylene black were all of analytical grade and used as received.
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