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Planapo 20 0.75 na objective

Manufactured by Nikon
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Sourced in United States, Japan

The PlanApo 20x/0.75 NA objective is a high-quality microscope objective from Nikon. It features a 20x magnification and a numerical aperture of 0.75, providing excellent optical performance and resolution. The objective is designed with a plan-apochromatic optical configuration for flat, distortion-free images across the entire field of view.

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Lab products found in correlation

Black 96 well clear imaging plates, by Greiner (2 mentions) Imagexpress micro xl automated bioimager, by Molecular Devices (1 mentions) Imagexpress micro xl automated, by Molecular Devices (1 mentions) Eclipse ti epifluorescence microscope, by Nikon (1 mentions)

Spelling variants of this product

40 × 0.75 NA objective (9 citations) ×20 PlanApo objective (5 citations) PlanApo NA 0.75 objective (3 citations)

5 protocols using planapo 20 0.75 na objective

1

High-resolution Imaging Protocols for smFISH

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High-quality/high-resolution imaging for smFISH was performed on a Cytiva DVLive epifluorescence image restoration microscope using an Olympus PlanApo 60×/1.42 NA objective and a 1900×1900 sCMOS camera. Z stacks ( 0.25μm ) covering the whole nucleus ( ∼10μm ) were acquired before applying a conservative restorative algorithm for quantitative image deconvolution.
High-throughput imaging for the QC analysis and follow-up experiments was performed on two different instruments: a) a Cytiva DVLive epifluorescence image restoration microscope using an Olympus PlanApo 20×/0.75 NA objective and a sCMOS camera; and, b) a Vala Sciences IC-200 high-throughputhigh-throughput epifluorescence microscope using a Nikon PlanApo 20×/0.75 NA objective and a sCMOS camera. Z stacks ( 0.75μm steps) covering the whole nucleus ( ∼10μm ) were acquired for all instruments. For the DVLive, images were deconvolved using a conservative restorative algorithm. Max intensity projections were generated and used for image analysis.
Stossi F., Singh P.K., Mistry R.M., Johnson H.L., Dandekar R.D., Mancini M.G., Szafran A.T., Rao A.U, & Mancini M.A. (2022). Quality Control for Single Cell Imaging Analytics Using Endocrine Disruptor-Induced Changes in Estrogen Receptor Expression. Environmental Health Perspectives, 130(2), 027008.
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2

Live-cell Imaging of Nuclear Hoechst Intensity

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Cells were seeded at a density of 7000 cells/well in 96-well flat bottom black imaging plates (#655090; Greiner Bio-One™). Cells were allowed to attach for 8 h and then treated with 1 μM of the selected KI, 10 μM verapamil, or DMSO as negative control. Each condition was tested in triplicate wells. 24 h after seeding, 1 μg/mL Hoechst33342 was added followed by an additional 2-h incubation. Subsequently, all wells were aspirated and received fresh medium including the respective inhibitors, without Hoechst33342 and plates were placed in a Nikon Eclipse Ti confocal microscope equipped with an automated stage, temperature and CO2-controlled incubator for live imaging and a Plan Apo ×20/0.75 NA objective (Nikon Instruments Inc., Melville, NY, USA). Total intensity of nuclear Hoechst33342 was calculated with CellProfiler [28 (link)] after watershed segmentation in FIJI-ImageJ [29 (link)]. The intensity values of three images from three replicate wells were averaged for each condition. Values of experimental groups were normalized to those of the DMSO control group.
Bergonzini C., Gregori A., Hagens T.M., van der Noord V.E., van de Water B., Zweemer A.J., Coban B., Capula M., Mantini G., Botto A., Finamore F., Garajova I., McDonnell L.A., Schmidt T., Giovannetti E, & Danen E.H. (2024). ABCB1 overexpression through locus amplification represents an actionable target to combat paclitaxel resistance in pancreatic cancer cells. Journal of Experimental & Clinical Cancer Research : CR, 43, 4.
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3

High-Content Imaging of Cell Stress

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In total, 5 x 103 U2OS cells were seeded into black 96-well µclear imaging plates (Greiner Bio-One) and allowed to adapt for 24 h. Thereafter the cells were treated with the DTT compounds and respective controls and incubated for additional 6 or 24 h before either 1 µM of DAPI or a mixture of 1 µM Hoechst, and 1 µM propidium iodide were added immediately before monitoring the uptake of the exclusion dye in a minimum of four view fields per well by means of an ImageXpress micro XL automated bioimager (Molecular Devices) equipped with a PlanApo 20 × /0.75 NA objective (Nikon, Tokyo, Japan).
Zhou H., Mondragón L., Xie W., Mauseth B., Leduc M., Sauvat A., Gomes-da-Silva L.C., Forveille S., Iribarren K., Souquere S., Bezu L., Liu P., Zhao L., Zitvogel L., Sveinbjørnsson B., Eksteen J.J., Rekdal Ø., Kepp O, & Kroemer G. (2018). Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals. Cell Death & Disease, 9(11), 1086.
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4

High-Content Imaging of Cell-Based DTT Assay

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In total, 5 x 103 U2OS cells were seeded into black 96-well µclear imaging plates (Greiner Bio-One) and allowed to adapt for 24 h. Thereafter, the cells were treated with DTT compounds and respective controls and incubated for additional 6 or 24 h before fixation in 3.7% (w/v) paraformaldehyde (PFA) in PBS supplemented with 1 µM Hoechst 33342 for 20 min. Upon fixation, cells were permeabilized with 0.1% Triton in PBS for 10 min at RT. Unspecific binding was blocked with 2% BSA in PBS for 10 min at RT followed by primary antibody diluted in BSA 2% following the manufactures recommendations overnight on an orbital shaker at 4 °C. The cells were rinsed twice and stained with AlexaFluor-coupled secondary antibodies for 1 h at RT, rinsed twice and subjected to imaging using an ImageXpress micro XL automated bioimager (Molecular Devices) equipped with a PlanApo 20 × /0.75 NA objective (Nikon).
Zhou H., Mondragón L., Xie W., Mauseth B., Leduc M., Sauvat A., Gomes-da-Silva L.C., Forveille S., Iribarren K., Souquere S., Bezu L., Liu P., Zhao L., Zitvogel L., Sveinbjørnsson B., Eksteen J.J., Rekdal Ø., Kepp O, & Kroemer G. (2018). Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals. Cell Death & Disease, 9(11), 1086.
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5

Quantifying Hair Follicle Orientation

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Full embryonic back skin images were acquired by tiling 20x images obtained on a Nikon Eclipse Ti epifluorescence microscope using a Plan Apo 20/0.75NA objective. NIS Elements software was used to tile and measure follicle angles. A reference angle was set along the anterior posterior axis and a line was drawn over each follicle towards its base. The difference between the two angles provides the orientation of the hair follicle.
Cetera M., Leybova L., Woo F.W., Deans M, & Devenport D. (2017). Planar cell polarity-dependent and independent functions in the emergence of tissue-scale hair follicle patterns. Developmental biology, 428(1), 188-203.
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