Insulin

Manufactured by InvivoGen

Insulin is a lab equipment product that functions as a protein hormone. It is responsible for regulating blood sugar levels by facilitating the absorption of glucose into cells.

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Lab products found in correlation

Retro x concentrator, by Takara Bio (1 mentions) Retro x universal packaging system, by Takara Bio (1 mentions) Dmem f12 glutamax supplement, by Thermo Fisher Scientific (1 mentions) Polybrene, by Merck Group (1 mentions) Puromycin, by InvivoGen (1 mentions) Hek293 cells, by Leibniz Institute DSMZ (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Normocin, by InvivoGen (1 mentions) Hek293 cells, by InvivoGen (1 mentions)

2 protocols using insulin

Generating NTCP-Expressing Cell Lines

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To generate human NTCP-expressing cells, Daudi, HepG2, or Huh7 cells were transduced with a retroviral vector encoding NTCP or Flag tag-NTCP with 10 μg/mL of Polybrene (Sigma-Aldrich, St. Louis, MO). The retroviral vectors were generated by modification of a previously described method (31 (link)). Briefly, the retroviral expression plasmid and the envelope expression plasmid p10A1 were cotransfected into GP2-293 cells using the Retro-X universal packaging system (TaKaRa Bio Inc., Shiga, Japan). At 2 days posttransfection, the culture supernatants were filtered through 0.45-μm filters and then concentrated using a Retro-X concentrator (TaKaRa Bio USA). At 48 h postinfection, cells were subjected to an immunofluorescence assay or a viral infection assay. HepG2-NTCP and HepG2/Flag-NTCP cells were maintained in DMEM/F-12, GlutaMAX supplement (catalog no. 10565018; Thermo Fisher Scientific) supplemented with 10% fetal calf serum (FCS) in the presence of 50 μM hydrocortisone, 5 μg/mL of insulin, and puromycin (0.5 μg/mL; InvivoGen).

Takemori T., Sugimoto-Ishige A., Nishitsuji H., Futamura Y., Harada M., Kimura-Someya T., Matsumoto T., Honma T., Tanaka M., Yaguchi M., Isono K., Koseki H., Osada H., Miki D., Saito T., Tanaka T., Fukami T., Goto T., Shirouzu M., Shimotohno K, & Chayama K. (2022). Establishment of a Monoclonal Antibody against Human NTCP That Blocks Hepatitis B Virus Infection. Journal of Virology, 96(5), e01686-21.

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Isolation and Culture of Primary Podocytes

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Primary podocytes were isolated from 10 days old pups of the different genotypes essentially as described (Rastaldi et al., 2006 (link)) and grown in Dulbecco's modified Eagle medium (DMEM) F12 supplemented with 10% FBS, 5 μg/ml transferrin,10⁻⁷ M hydrocortisone, 5 ng/ml sodium selenite, 0.12 U/ml insulin, 5 μg/ml normocin (Invivogen, San Diego, CA), 100 μg/ml penicillin, 100 μg/ml streptomycin and 2mM L-glutamine (Sigma–Aldrich, Taufkirchen, Germany). HEK293 cells (DSMZ, Braunschweig, Germany) and murine embryonic fibroblasts (Vogt et al., 2003 (link)) were grown in DMEM and 10% FBS. The growth medium of HEK293 cells stably expressing TRPC6-HA was supplemented with 800 μg/ml G418 (Invivogen).

Kalwa H., Storch U., Demleitner J., Fiedler S., Mayer T., Kannler M., Fahlbusch M., Barth H., Smrcka A., Hildebrandt F., Gudermann T, & Dietrich A. (2015). Phospholipase C Epsilon (PLCε) Induced TRPC6 Activation: A Common but Redundant Mechanism in Primary Podocytes. Journal of cellular physiology, 230(6), 1389-1399.

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