Vt1000s vibratome

Manufactured by Leica

Sourced in Germany, United States, United Kingdom, France

The VT1000S vibratome is a precision instrument used for cutting thin sections of biological samples. It utilizes a vibrating blade to slice through samples with minimal damage, enabling high-quality sectioning for microscopy and analysis.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (12 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (9 mentions) Lsm 700 confocal microscope, by Zeiss (8 mentions) Vectashield with dapi, by Vector Laboratories (8 mentions) Paraformaldehyde (pfa), by Merck Group (7 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (7 mentions) Vectashield, by Vector Laboratories (6 mentions) Lsm 700, by Zeiss (6 mentions) Fluoromount g, by Southern Biotech (6 mentions) Vs120 microscope, by Olympus (5 mentions) Low melting point agarose, by Thermo Fisher Scientific (5 mentions) Vectastain elite abc kit, by Vector Laboratories (5 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (5 mentions) Low melting point agarose, by Merck Group (4 mentions) Lsm 780 confocal microscope, by Zeiss (4 mentions) Fv1000, by Olympus (4 mentions) Alexa fluor 647, by Thermo Fisher Scientific (4 mentions) Lsm 880 confocal microscope, by Zeiss (4 mentions) Vs120 slide scanning microscope, by Olympus (3 mentions) Mouse anti neun, by Abcam (3 mentions)

Spelling variants of this product

VT1000S (1652 citations) Vibratome (897 citations) Vibratome VT1000S (146 citations) VT1000 A vibratome (3 citations) VT1000 S Vibrating-blade vibratome (2 citations)

491 protocols using vt1000s vibratome

Perfusion and Fixation of Mouse Brains

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The mouse was anesthetized by isoflurane until there was no toe-pinch reflex. Mice were then transcardially perfused with aCSF (125 mM NaCl, 26 mM NaHCO₃, 1.25 mM NaH₂PO₄, 2.5 mM KCl, 26 mM glucose, 1 mM MgCl₂ and 2 mM CaCl₂ (all chemicals were from Sigma-Aldrich) at the flow rate of 10 ml/min for 2 min to remove blood, followed with 4% paraformaldehyde (Electron Microscopy Sciences), 0.1% glutaraldehyde (Electron Microscopy Sciences) in 1 × PBS for 3 min for fixation. Brains were dissected and then post-fixed in the same fixative on a rotator overnight at 4°C. Brains were sectioned into 50-μm or 120-μm coronal sections using a Leica VT1000 S vibratome and stored in the same fixative at 4°C.
For ECS-preserving perfusion, the detailed protocol was described in ²⁷. In brief, mice were anesthetized by isoflurane, transcardially perfused with aCSF at the flow rate of 10 ml/min for 2 min to remove blood, followed with 15 w/v% mannitol (Sigma-Aldrich) aCSF solution for 1 min, 4 w/v% mannitol aCSF solution for 5 min, and 4 w/v% mannitol, 4% paraformaldehyde in 1 × PBS for 5 min. Brains were dissected out and then post-fixed in the same fixative for 3 h on a rotator at 4 °C. Brains were sectioned into 50-μm coronal sections using a Leica VT1000 S vibratome, and then store in in 1 × PBS at 4 °C.

Han X., Lu X., Li P.H., Wang S., Schalek R., Meirovitch Y., Lin Z., Adhinarta J., Berger D., Wu Y., Fang T., Meral E.S., Asraf S., Ploegh H., Pfister H., Wei D., Jain V., Trimmer J.S, & Lichtman J.W. (2023). Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex. Research Square.

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Lymph Node Slicing and Incubation Protocol

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LNs were embedded in a 6% w/v low-melting agarose (Thomas Scientific) using 35 mm Petri dishes. LNs were placed into the agarose with the wide side of the node facing up and a 10 mm biopsy punch (Robbins Instruments) was used to punch out the embedded tissues. The gel pieces were oriented so that the tissue was at the top of the gel piece and glued (CA 221 fast curing glue, Best Klebstoffe) onto the specimen holder of a Leica VT1000S vibratome. The Leica VT1000S vibratome was set to a speed of 3.9 mm s⁻¹, frequency set to 0.3 Hz, and amplitude set to 0.6 mm and up to 4 LNs were sliced simultaneously. 1× PBS was placed into the buffer tray and ice was continually placed in the area surrounding the buffer tray to ensure the 1× PBS was cold throughout the duration of the slicing. LN slices were collected using a brush and placed into RPMI (Thermo Fisher) supplemented with 10% FBS (Sigma), 1× l-glutamine (Corning), 50 U mL⁻¹ Pen/Strep (Sigma), 50 μM beta-mercaptoethanol (Thermo Fisher), 1 mM sodium pyruvate (Thermo Fisher), 1× nonessential amino acids (Fisher Scientific), and 20 mM HEPES (GE Healthcare). LN slices were incubated at 37 °C with 5% CO₂ in media for at least 1 h prior to performing multiple particle tracking.

Ringuet H., Akoua-Koffi C., Honore S., Varnerot A., Vincent V., Berche P., Gaillard J.L, & Pierre-Audigier C. (1999). hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria. Journal of Clinical Microbiology, 37(3), 852-857.

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Tissue Fixation and Sectioning for Microscopy

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After being anesthetized with thiopental (120 mg/kg; Sandoz), adult male mice were transcardially perfused with 0.9% NaCl followed by an ice-chilled fixative solution. For fluorescence microscopy, a fixative solution composed of 2% paraformaldehyde (PFA) and 15% saturated picric acid in 0.1 m phosphate buffer (PB) was used. On the other hand, for horseradish peroxidase (HRP) reactions, the fixative solution contained 4% PFA and 15% saturated picric acid in 0.1 m PB, pH 7.4. The same fixative was also used for pre-embedding electron microscopy (EM) with the addition of 0.05% glutaraldehyde. Brains were immediately removed from the skull, washed in 0.1 m PB, and sliced coronally in 50-μm-thick sections for light microscopy or in 70-μm-thick sections for EM on a Leica VT1000S vibratome (Leica Microsystems). Sections were stored in 0.1 m PB containing 0.05% sodium azide at 6°C until further use. Brains processed for EM were first cut in 6–8-mm-thick blocks, cryoprotected in a solution of 20% sucrose in 0.1 m PB, and freeze-thawed once before sectioning.

Rhomberg T., Rovira-Esteban L., Vikór A., Paradiso E., Kremser C., Nagy-Pál P., Papp O.I., Tasan R., Erdélyi F., Szabó G., Ferraguti F, & Hájos N. (2018). Vasoactive Intestinal Polypeptide-Immunoreactive Interneurons within Circuits of the Mouse Basolateral Amygdala. The Journal of Neuroscience, 38(31), 6983-7003.

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Mouse Brain Sectioning and Imaging

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For images in Figure 1B, mice were deeply anesthetized at P30 with isoflurane and perfused transcardially with 4% paraformaldehyde in phosphate buffered saline (PBS). Brains were postfixed overnight, washed in PBS and 40 μm parasagittal sections were cut with a Leica VT1000S vibratome (Leica Instruments). They were then mounted on superfrost slides, dried and covered with ProLong antifade reagent containing DAPI (Molecular Probes) followed by a coverslip. Whole sections were imaged with an Olympus VS120 slide-scanning microscope.

Chen Y., Granger A.J., Tran T., Saulnier J.L., Kirkwood A, & Sabatini B.L. (2017). Endogenous Gαq-coupled Neuromodulator Receptors Activate Protein Kinase A. Neuron, 96(5), 1070-1083.e5.

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Immunolabeling of Rph3a in Rat Brain

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Three Sprague–Dawley male rats (∼6-week-old) of ∼150 g were used. After terminal anaesthesia was induced by brief inhalation of isoflurane (0.05% in air), followed by an intramuscular injection of ketamine (100 mg kg⁻¹) and xylazine (10 mg kg⁻¹), rats were intracardially perfused with 4% PFA and 0.1% glutaraldehyde in PBS (0.1 M, pH 7.2), and brain sections (100 μm) were cut on a Leica VT1000S vibratome (Leica Microsystems)19 (link)60 (link). The sections were immunolabelled with rabbit anti-Rph3a polyclonal antibody (1:100; ab68857, Abcam), followed by a biotinylated secondary antibody, ABC Elite Kit (Vector Laboratories), and the peroxidase reaction was revealed by ImmPACT VIP substrate Kit (Vector Laboratories). Then the sections were osmicated, dehydrated and flat embedded in Durcupan resin (Sigma-Aldrich). Ultrathin sections (70–90 nm) were countercoloured with uranyl acetate and lead citrate. Control experiments, in which the primary antibody was omitted, resulted in no immunoreactivity. The sections were visualized with a Philips CM100 transmission electron microscope (FEI) at 100 kV. The images were captured with an AMT XR40 4 megapixel side mounted CCD camera at a magnification between × 7,900 and × 92,000.

Stanic J., Carta M., Eberini I., Pelucchi S., Marcello E., Genazzani A.A., Racca C., Mulle C., Di Luca M, & Gardoni F. (2015). Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nature Communications, 6, 10181.

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Fluorescent Microscopy of Brain Tissue

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Fluorescent microscopy was conducted on freshly isolated perfused brain tissue that was sliced into 300 μm with a Leica VT1000s vibratome (Leica Microsystems) and plated onto uncoated glass slides. Brain slices were immediately imaged with a Nikon Eclipse TE2000-E inverted fluorescent microscope and analyzed using MetaMorph Microscopy Automation and Image Analysis Software.

Flores C., Pham C., Snyder D., Yang S., Sanchez-Perez L., Sayour E., Cui X., Kemeny H., Friedman H., Bigner D.D., Sampson J, & Mitchell D.A. (2015). Novel role of hematopoietic stem cells in immunologic rejection of malignant gliomas. Oncoimmunology, 4(3), e994374.

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Immunofluorescent Characterization of 3D iPSC-Derived Eyecups

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3D iPSC-derived eyecups were embedded in 4% agarose, sectioned at a
thickness of 100μm using a Leica VT1000 S vibratome (Leica Microsystems,
Wetzlar, Germany) and labeled with primary antibodies targeted against: mouse anti-SOX2
(#MAB2018; 1:1000; R&D Systems, Minneapolis, MN), rabbit anti-PAX6
(#901301; 1:1000; BioLegend, San Diego, CA), goat anti-biotinylated-OTX2
(#BAF1979; 1:500; R&D Systems, Minneapolis, MN), rabbit anti-Ki67
(#ab15580; 1:500; Abcam, Cambridge, MA), rabbit anti-TUJ1 (neuron-specific class
III beta-tubulin; #T2200; 1:500; Sigma-Aldrich; 1:500), goat
anti-biotinylated-NRL (#BAF2945; 1:500; R&D Systems), mouse anti-HuC/D
(#A-21271; 1:500; Thermo Fisher Scientific, Waltham, MA) and rabbit
anti-recoverin (#AB5585; 1:2000; EMD Millipore, Billerica, MA). To detect
F-actin, eyecups were stained with Alexa Fluor® 488 Phalloidin (Life
Technologies, Madison, WI; #A12379; 1:500). Primary antibodies were detected
using fluorescently-conjugated Alexa Fluor® secondary antibodies (Life
Technologies). Cell nuclei were counterstained using DAPI. Sectioned eyecups were imaged
using a Leica DM 2500 SPE confocal microscope (Leica Microsystems).

Small K.W., DeLuca A.P., Whitmore S.S., Rosenberg T., Silva-Garcia R., Udar N., Puech B., Garcia C.A., Rice T.A., Fishman G.A., Héon E., Folk J.C., Streb L.M., Haas C.M., Wiley L.A., Scheetz T.E., Fingert J.H., Mullins R.F., Tucker B.A, & Stone E.M. (2015). North Carolina Macular Dystrophy is caused by dysregulation of the retinal transcription factor PRDM13. Ophthalmology, 123(1), 9-18.

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Mouse Hippocampal Slice Preparation

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Transverse hippocampal slices were prepared from mice (P21–P28). Animals were anesthetized by inhalation of isoflurane, and killed by decapitation. The cerebral hemispheres were quickly removed and placed into ice-cold choline-based artificial cerebrospinal fluid (choline-ACSF) consisting of (in mM): 110 choline-Cl, 25 NaHCO₃, 1.25 Na₂HPO4, 2.5 KCl, 7 MgCl₂, 25 glucose, 0.5 CaCl₂, 11.6 ascorbic acid, 3.1 pyruvic acid and equilibrated with 95% O₂ / 5% CO₂. Blocking cuts were made and tissue transferred to a slicing chamber containing choline-ACSF. Slices (300 μm) were cut with a LeicaVT1000 s vibratome (Leica Instruments) and transferred to a holding chamber containing ACSF consisting of (in mM): 127 NaCl, 25 NaHCO₃, 1.25 Na₂HPO₄, 2.5 KCl, 2 CaCl₂, 1 MgCl₂, 25 glucose, saturated with 95% O₂ / 5% CO₂, and supplemented with 250 μM kynurenic acid (KYN) and 0.2 μM TTX. Slices were incubated at 31°C for 30 min and then maintained at room temperature (RT) for the duration of experiment up to 6 h.

Brigidi G.S., Hayes M.G., Santos N.P., Hartzell A.L., Texari L., Lin P.A., Bartlett A., Ecker J.R., Benner C., Heinz S, & Bloodgood B.L. (2019). Genomic decoding of neuronal depolarization by stimulus-specific NPAS4 heterodimers. Cell, 179(2), 373-391.e27.

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Preparation of Mouse Brain Slices

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Animals were anesthetized by intraperitoneal injection of Sagatal (sodium pentobarbitone, 100 mg/kg, Rhône Mérieux Ltd., Harlow, United Kingdom). When pedal reflexes were abolished, the animals were perfused intracardially with chilled (5°C) oxygenated artificial cerebrospinal fluid (ACSF), in which the sodium chloride had been replaced by iso-osmotic sucrose. This sucrose-ACSF contained (in mM): 225 sucrose, 3 KCl, 1.25 NaH₂PO₄, 24 NaHCO₃, 6 MgSO₄, 0.5 CaCl₂, and 10 glucose (pH: 7.4). Horizontal slices (350 μm) of the mouse brain were cut in chilled sucrose-ACSF, using a Leica VT1000S vibratome (Leica Microsystems, Milton Keynes, United Kingdom). Slices containing the ventral hippocampus were stored at room temperature at the interface between recording ACSF and humidified carbogen (95% O₂–5% CO₂), until transferred to the Haas-type recording chamber. The recording ACSF contained (in mM): 126 NaCl, 3 KCl, 1.25 NaH₂PO₄, 24 NaHCO₃, 2 MgSO₄, 2 CaCl₂, and 10 Glucose (pH: 7.4).

Wang Y., Jin Y.K., Guo T.C., Li Z.R., Feng B.Y., Han J.H., Vreugdenhil M, & Lu C.B. (2022). Activation of Dopamine 4 Receptor Subtype Enhances Gamma Oscillations in Hippocampal Slices of Aged Mice. Frontiers in Aging Neuroscience, 14, 838803.

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Characterization of mGluR5 and D1 in Mouse Brain

Cited 1 time

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For immunohistochemical characterization, mice were first deeply anaesthetized with thiopental sodium (150 mg/kg, i.p.) and transcardially perfused with a fixative (4% paraformaldehyde + 15% picric acid in 0.1 M phosphate-buffer (PB), pH 7.2–7.4). Following brain extraction, coronal sections were cut (50 μm) on a Leica VT1000S vibratome (Leica Microsystems, Vienna, Austria) and immunostained against mGluR5 and D1, based on previously described procedures [76 (link)]. A rabbit antibody against mGluR5 (Frontier Institute, Hokkaido, Japan; AB_2571802) and a goat antibody against D1 (Frontier Institute, AB_2571594) were diluted 1:1000 in 2% normal goat serum (NGS), 0.1% Triton X-100 in Tris-buffered saline (TBS; pH 7.4) and sections incubated for 48 h at 6 °C. Sections were then incubated overnight with the respective secondary antibodies (anti-goat Alexa Fluor™488, 1:1000, Jackson ImmunoResearch Europe Ltd.; anti-rabbit Cy3, 1:500, Invitrogen, ThermoFisher Scientific, Vienna, Austria). After three washing steps with TBS, sections were finally mounted onto gelatin-coated slides and coverslipped with Vectashield (Vector Laboratories, Burlingame, US). Immunofluorescent sections were examined using a Zeiss AxioImager M1 microscope or a confocal laser-scanning microscope (SP5, Zeiss, Oberkochen, Germany) for low and high-resolution image acquisition, respectively.

Zangrandi L., Schmuckermair C., Ghareh H., Castaldi F., Heilbronn R., Zernig G., Ferraguti F, & Ramos-Prats A. (2021). Loss of mGluR5 in D1 Receptor-Expressing Neurons Improves Stress Coping. International Journal of Molecular Sciences, 22(15), 7826.

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