Brexpiprazole

Anti-survivin (#2808), anti-p-AKT (Ser473 #9271, Thr308 #9275), anti-AKT (#9272), anti-p-mTOR (#2974), anti-mTOR (#2972), anti-p-ERK (#9101), anti-ERK (#4695), and anti-EGFR (#4267) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Anti-β-actin (A1978) antibody was from Sigma (St. Louis, MO, USA). Osimertinib and YM155 were purchased from Chemscene LLC. (Monmouth Junction, NJ, USA), and dissolved in dimethyl sulfoxide (DMSO) to 10 mM and 20 μM, respectively, as stock solutions. Brexpiprazole was from Cayman Chemical Company (Ann Arbor, MI, USA) and was dissolved in DMSO to 10 mM as a stock solution.

Anti-Sox2 (#3579), anti-Nanog (#4903), anti-Bmi1 (#2830), and anti-Survivin (#2808) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Anti-β-actin (A1978) antibody was from Sigma (St. Louis, MO, USA). Anti-CD133 antibody (W6B3C1) was from Miltenyi Biotec (Germany). Gemcitabine and 5-fluorouracil (5-FU) were from Sigma, and were dissolved in DMSO to prepare 1 mM and 200 mM stock solutions, respectively. Brexpiprazole was from Cayman Chemical (Ann Arbor, MI, USA) and dissolved in DMSO to prepare a 10 mM stock solution.

Aripiprazole, Clozapine, Quetiapine, Olanzapine, Paliperidone, Risperidone and Rotenone (Abcam), DehydroAripiprazole (Santa Cruz Biotechnology), DidesmethylCariprazine (Clearsynth), Haloperidol, Iloperidone, Lurasidone, Zotepine (Sigma), Brexpiprazole and Cariprazine (Cayman Chemicals) were dissolved to desired stock concentrations in DMSO. Chlorpromazine (Sigma) was dissolved in distilled water, while Antimycin A, FCCP, Oligomycin A (Sigma) and Piericidin A (Cayman Chemicals) were dissolved in ethanol. All drug stock solutions were diluted into appropriate cell culture media to desired concentrations.