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Gaspak ez co2 sachets

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The BD GasPak EZ CO2 sachets are a laboratory product designed to generate a controlled carbon dioxide (CO2) atmosphere. The sachets provide a simple and convenient way to create the desired CO2 environment for various cell culture, microbiology, and other laboratory applications.

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GasPak EZ (56 citations) GasPak EZ sachets (6 citations) GasPaks (2 citations)

3 protocols using gaspak ez co2 sachets

1

Growth of C. jejuni under Microaerobic Conditions

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The C. jejuni strains used in this study (Table 1) were grown under microaerobic conditions (BD GasPak EZ CO2 sachets, Becton Dickinson) at 37°C on brain-heart infusion (BHI) agar containing 5% (v/v) sheep blood, supplemented when necessary with chloramphenicol at 20 µg ml-1.
Sałamaszyńska-Guz A., Rasmussen P.K., Murawska M, & Douthwaite S. (2022). Campylobacter jejuni Virulence Factors Identified by Modulating Their Synthesis on Ribosomes With Altered rRNA Methylation. Frontiers in Cellular and Infection Microbiology, 11, 803730.
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2

Culturing C. jejuni and E. coli Strains

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The bacterial strains and plasmids used in this study are listed in Table S1. C. jejuni strains were grown at 37°C on Mueller-Hinton (MH, bioMérieux) agar containing 5% (v/v) sheep blood under microaerobic conditions using BD GasPak EZ CO2 sachets (Becton Dickinson). Escherichia coli strains were grown at 37°C in Luria Bertani (LB) broth or agar (bioMérieux) supplemented with chloramphenicol at 20 μg ml−1 and/or kanamycin at 25 μg ml−1.
Sałamaszyńska-Guz A., Rose S., Lykkebo C.A., Taciak B., Bącal P., Uśpieński T, & Douthwaite S. (2018). Biofilm Formation and Motility Are Promoted by Cj0588-Directed Methylation of rRNA in Campylobacter jejuni. Frontiers in Cellular and Infection Microbiology, 7, 533.
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3

Cultivation of C. jejuni Strains

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The C. jejuni strains used in this study (Table 1) were grown under microaerobic conditions (BD GasPak EZ CO2 sachets, Becton Dickinson) at 37°C on brain–heart infusion (BHI) agar containing 5% (v/v) sheep blood, and in some cases supplemented with chloramphenicol at 20 μg/ml and/or kanamycin at 30 μg/ml.
Sałamaszyńska‐Guz A., Serafińska I., Bącal P, & Douthwaite S. (2020). Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA. Cellular Microbiology, 22(7), e13199.
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