For in vivo experiments, Stab1 KO (B6.129S2-Stab1tm1.1Cger 16) [12 (link)] were used. Homozygous littermates or purchased C57Bl/6J wildtype (Janvier Labs, Le Genest-Saint-Isle, France) served as Ctrl. Heterozygous mice were not used. All animals were hosted in single ventilated cages (Sealsafe plus DGM™, Techniplast, Buguggiate, Italy; Bedding H0234-20, Ssniff, Soest, Germany) in a 12 h/12 h day/night cycle under specific-pathogen-free conditions and fed ad libitum with a standard rodent diet (ssniff®®R/M-H autoclavable, V1534-000, Ssniff, Soest, Germany). For DNA extraction and genotyping, the KAPA HotStart Mouse Genotyping Kit (KK7352, Merck, Darmstadt, Germany) and primers (Metabion international AG, Planegg/Steinkirchen, Germany) were utilized (Supplementary Table S1 ).
Sealsafe plus dgm
Manufactured by Tecniplast
Sourced in Italy
The Sealsafe plus DGM™ is a ventilated animal housing system designed by Tecniplast. It provides a controlled microenvironment for the housing of laboratory animals. The system utilizes a dynamic gradient of airflow to maintain air quality and temperature within the cages.
Automatically generated - may contain errors
4 protocols using sealsafe plus dgm
1
In vivo Stab1 KO Mouse Protocol
2
In Vivo Metastasis Experiments on C57Bl/6 Mice
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For in vivo experiments female C57Bl/6 wildtype mice were purchased by Janvier. For metastasis experiments mice were age-matched and were used at least at 10 weeks of age. All animals were hosted in single ventilated cages (Sealsafe plus DGM™, Techniplast, Italy; Bedding H0234-20, Ssniff, Germany) in a 12 h/12 h day/night cycle under Specific-pathogen-free conditions and fed ad libitum with a standard rodent diet (ssniff®R/M-H autoclavable, V1534-000, Ssniff, Germany).
3
In vivo Mouse Metastasis Experiments
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For in vivo experiments female C57Bl/6 wildtype mice were purchased by Janvier. For metastasis experiments mice were age-matched and were used between 11 to 13 weeks of age. All animals were hosted in single ventilated cages (Sealsafe plus DGM™, Techniplast, Italy; Bedding H0234-20, Ssniff, Germany) in a 12 h/12 h day/night cycle under specific-pathogen-free conditions and fed ad libitum with a standard rodent diet (ssniff®R/M-H autoclavable, V1534-000, Ssniff, Germany).
4
Detailed Mouse Husbandry Protocol
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Mice used were between 11 and 14 weeks of age; sibling controls were used except for Wls‐rescue, for which background, age‐ and sex‐matched controls from the same facility were used. eYFP‐reporter animals were sacrificed at embryonic day (E)11.5 and 6 weeks. All animals were hosted in single ventilated cages (Sealsafe plus DGM [Techniplast, Italy] and Bedding H0234‐20 [Ssniff, Germany]) in a 12‐hour day/night cycle under specific pathogen‐free (SPF) conditions and fed ad libitum with a standard rodent diet (ssniffR/M‐H autoclavable, V1534‐000; Ssniff). Bisected kidney basins were used as enrichment. All animal experiments were approved by the animal ethics committee in Baden‐Wuerttemberg (Regierungspraesidium Karlsruhe).
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