RL95-2 cells were treated with radiotherapy, α-bisabolol or the coordination of both. Hoechst Staining Kit (C0003) was purchased from Beyotime (Shanghai, China) to detect the apoptotic cells. Experiment was carried out according to the instructions.
Hoechst staining kit c0003
Manufactured by Beyotime
Sourced in China
The Hoechst staining kit (C0003) is a laboratory product designed for DNA staining. It contains the Hoechst 33342 dye, which binds specifically to the minor groove of DNA, allowing for the visualization and quantification of DNA in various applications.
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3 protocols using hoechst staining kit c0003
1
Apoptosis Induction in RL95-2 Cells
2
Hoechst Nuclear Staining for Apoptosis
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A Hoechst staining kit (C0003) (Beyotime Biotechnology, Shanghai, China) was used to detect the morphological changes in cell nuclei. The results were visualized using an IX53 Olympus fluorescence microscope (Olympus, Tokyo, Japan) at ×400 magnification. Cell nuclei were stained bright blue (Hoechst-positive) were identified as apoptotic cells.
3
Berberine Biomarker Detection Protocol
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Berberine was purchased from MedChemExpress (Monmouth Junction, NJ) and dissolved in saline. Agents for PCR detection, including RNA Purified Total RNA Extraction Kit (RP1201) and super M-MLV reverse transcriptase (RP6502) were purchased from BioTeke (Beijing, China). Agents for western blotting detection, including PMSF lysis (P0013B), protein concentration determination kit (P0012), cell cycle detection kit (C1052), CCK-8 detection kit (C0037) and Hoechst staining kit (C0003) were purchased from Beyotime Biotechnology (Shanghai, China). Cell apoptosis detection kit (KGA106) was purchased from KeyGen BioTECH (Nanjing, China). Detection kits for malondialdehyde (MDA) (A003-1), glutathione (GSH) (A006-2), glutathione peroxidase (GSH-Px) (A005) and superoxide dismutase (SOD) (A001-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Detection kits for tumour necrosis factor-α (TNF-α) (EK3821/2), interleukin-1β (IL-1β) (EK301B1/2) and IL-6 (70-EK206) were purchased from Multi Sciences (Hangzhou, China).
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