Tumour samples were fixed in 3.7% PFA for 24 h at 4 °C, dehydrated with 70% EtOH for 24 h at 4 °C and subsequently rehydrated with demineralized water. The samples were then cut in sections of about 4 µm. Specimens were stained with haematoxylin and eosin and immunohistochemistry was performed using microwave pre-treatment of slides for antigen retrieval. Antibodies against Ki-67 (SP6, Thermo Fisher Scientific #RM-9106-S, clone SP6, 1:200) and puromycin (Merck Millipore, clone 12D10, 1:15,000) were applied, in conjunction with goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies (DAKO) and visualized by DAB reaction. To evaluate the stainings, positive cells were counted using ImageJ.
Goat anti rabbit horseradish peroxidase hrp conjugated antibodies
Manufactured by Agilent Technologies
Goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies are a type of secondary antibody used in various immunoassay techniques. These antibodies are produced by immunizing goats with rabbit immunoglobulins and subsequently conjugating them with the enzyme horseradish peroxidase. This conjugation allows for the detection and quantification of target analytes in samples when used in conjunction with a primary antibody specific to the analyte of interest.
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2 protocols using goat anti rabbit horseradish peroxidase hrp conjugated antibodies
1
Immunohistochemical Analysis of Tumor Samples
2
Immunohistochemical Analysis of Tumor Samples
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