Cells were stained to verify the purity of isolated B cells or to define B-cell phenotype by using anti-CD38, anti-CD24, anti-CD27, anti-CD71, anti-CD80, anti-CD86, anti-CD20 (Beckman Coulter, Brea, CA, USA), PD-1 (Miltenyi Biotech), PD-L1 and anti-TIM-1 (eBiosciences) antibodies. For intracellular labelling of IL-10 (Miltenyi Biotech), the B-cell cultures were supplemented with Phorbol 12-myristate 13-acetate (PMA; 10 ng/mL) + Ionomycin (0.25 µg/mL, both from Sigma Aldrich) during 5 h and GolgiStop (BDbiosciences; Franklin Lakes, NJ, USA) during the last 4 h of incubation. Then, cells were washed, surface-stained, stained for viable cells with Fixable Viability Dye eFluor450 (eBiosciences), fixed/permeabilised (Cytofix/Cytoperm, BDbiosciences) and stained for intracellular IL-10. Cells were then analysed by flow cytometry using a Gallios cytometer (Beckman Coulter), and data were analysed using the Kaluza software (Beckman Coulter).
Anti cd80
Manufactured by Beckman Coulter
Sourced in United States, Germany
Anti-CD80 is a laboratory product that detects the presence of the CD80 protein, also known as B7-1, on the surface of cells. CD80 is a costimulatory molecule involved in the activation of T cells. This product can be used in various cell analysis and immunological research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd86, by Beckman Coulter (3 mentions)
Cytofix cytoperm, by BD (1 mentions)
Fixable viability dye efluor 450, by Thermo Fisher Scientific (1 mentions)
Gallios cytometer, by Beckman Coulter (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Golgistop, by BD (1 mentions)
Pd l1, by Thermo Fisher Scientific (1 mentions)
Kaluza software, by Beckman Coulter (1 mentions)
Il 10, by Miltenyi Biotec (1 mentions)
Anti cd56, by Beckman Coulter (1 mentions)
Anti cd4, by Beckman Coulter (1 mentions)
Anti cd3, by Beckman Coulter (1 mentions)
Anti hla dr, by Beckman Coulter (1 mentions)
Epics xl mcl, by Beckman Coulter (1 mentions)
Anti cd45, by Beckman Coulter (1 mentions)
Anti tcr vβ11, by Beckman Coulter (1 mentions)
Anti cd8, by Beckman Coulter (1 mentions)
Anti mhc class 2, by Miltenyi Biotec (1 mentions)
3 protocols using anti cd80
1
Flow Cytometric Analysis of B-Cell Phenotype
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Surface phenotypes were determined using an Epics XL MCL (Beckman Coulter, Brea, CA, USA). The following monoclonal antibodies (mAbs) were purchased from Beckman Coulter: anti‐CD3, anti‐CD4, anti‐CD8, anti‐Vγ9TCR, anti‐CD14, anti‐CD25, anti‐CD45, anti‐CD54, anti‐CD56, anti‐HLA‐DR, anti‐CD40, anti‐CD80, anti‐CD86, anti‐CD11c, anti‐CD36, mouse immunoglobulin (Ig)G1, mouse IgG2 and mouse IgG2b mAbs. Anti‐HLA‐class 1 and anti‐CCR7 mAbs were purchased from Beckton Dickinson (San Jose, CA, USA) and R&D Systems (Minneapolis, MN, USA), respectively. Anti‐TCR Vα24TCR and anti‐TCR Vβ11 mAbs were purchased from Beckman Coulter (Villepinte, France). Anti‐human CD273 (PD‐L2) and CD274 (PD‐L1) mAbs were purchased form eBioscience (San Diego, CA, USA). Anti‐human CD152 (CTLA‐4) and CD279 (PD‐1) mAbs were purchased from BioLegend (San Diego, CA, USA). Anti‐FoxP3 mAb for intracellular staining was purchased from BD Biosciences (Tokyo, Japan). All mAbs were conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), antigen‐presenting cells (APC), extracellular domain (ECD), proprotein convertase (PC)5 or PC7. Z was purchased from Novartis Pharmaceuticals (Basel, Switzerland) and G from Funakoshi Co. Ltd (Tokyo, Japan).
3
Isolation and Phenotypic Analysis of Tumor-Infiltrating Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Splenocytes were isolated at 48 hours after the last administration of polyplex micelles to subcutaneous tumor models. The cells were then subjected to flow cytometric analysis using PE-conjugated anti-CD11c (Miltenyi Biotec Gmbh, Bergisch Gladbach, Germany), FITC-conjugated anti-CD11b (Abcam), anti-CD80, anti-CD86 (Beckman Coulter, Fullerton, CA), and anti-MHC-Class II (Miltenyi Biotec Gmbh) monoclonal antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!