Uridine 5 triphosphate

Manufactured by Merck Group

Sourced in United States

Uridine 5'-triphosphate is a nucleotide that is a naturally occurring pyrimidine nucleoside triphosphate. It serves as a precursor for RNA synthesis and is involved in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Adenosine 5 triphosphate, by Merck Group (3 mentions) Uridine 5 diphosphate, by Merck Group (3 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) 5 fluoro 2 deoxyuridine, by Merck Group (2 mentions) Adenosine 5 diphosphate, by Merck Group (2 mentions) Guanosine 5 triphosphate, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Neurobasal medium, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Cast eij, by Jackson ImmunoResearch (1 mentions) Oxotremorine m, by Merck Group (1 mentions) Rapamycin, by LC Laboratories (1 mentions) 2 thio utp, by Bio-Techne (1 mentions) Glutamax, by Merck Group (1 mentions) Oligo dt 18, by Thermo Fisher Scientific (1 mentions) Collagenase type 1, by Thermo Fisher Scientific (1 mentions) Type 1 collagen, by BD (1 mentions) Dmem f12, by Merck Group (1 mentions) B27 and n2 supplement, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Uridine (351 citations) Uridine triphosphate (5 citations) Uridine 5’-triphosphate (UTP) (3 citations) Uridine 5′-Triphosphate trisodium salt hydrate (2 citations)

8 protocols using uridine 5 triphosphate

Hybrid mouse cortical neuron cultures

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary mouse cortical neuron cultures were prepared as previously described from E14.5 pregnant C57BL/6J (Cat. #000664, Jackson) dams crossed to CAST/EiJ (Cat. #000928, Jackson) males18 (link). Hybrid cultures are thought to better model genetic variation associated with human populations58 (link). Dissociated cells were placed in multiwell plates coated with poly-D-lysine (0.1 mg ml⁻¹) in Neurobasal medium (Life Technologies) containing 5% fetal bovine serum (Gibco), B27 (17504-044, Invitrogen), Antibiotic-Antimycotic (15240-062, Invitrogen) and GlutaMAX (35050-061, Invitrogen). At DIV 3, a half medium change was performed with feeding medium identical to the plating medium except that we omitted fetal bovine serum and included 4.84 μg ml⁻¹ uridine 5′-triphosphate (U6625, Sigma-Aldrich) and 2.46 μg ml⁻¹ 5-fluoro-2′-deoxyuridine (F0503, Sigma-Aldrich) to inhibit mitosis in dividing cells.

Pearson B.L., Simon J.M., McCoy E.S., Salazar G., Fragola G, & Zylka M.J. (2016). Identification of chemicals that mimic transcriptional changes associated with autism, brain aging and neurodegeneration. Nature Communications, 7, 11173.

+ Open protocol

+ Expand

Electrophysiological Recordings of KCNQ2/3 Channels

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments used Ringer’s solution containing 160 mM NaCl, 2.5 mM KCl, 2 mM CaCl₂, 1 mM MgCl₂, 10 mM Hepes, and 8 mM glucose, pH 7.4 (NaOH). Intracellular pipette solution for KCNQ2/3 recordings contained 175 mM KCl, 5 mM MgCl₂, 5 mM Hepes, 0.1 mM K₄BAPTA, 3 mM Na₂ATP, and 0.1 mM Na₃GTP, adjusted with NaOH to pH 7.2. Rapamycin (LC Laboratories), uridine-5′-triphosphate, and oxotremorine-M (both Sigma-Aldrich) were applied in the superfusate.

Dickson E.J., Jensen J.B., Vivas O., Kruse M., Traynor-Kaplan A.E, & Hille B. (2016). Dynamic formation of ER–PM junctions presents a lipid phosphatase to regulate phosphoinositides. The Journal of Cell Biology, 213(1), 33-48.

+ Open protocol

+ Expand

Compound 89 Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Compound 89, also known as 2-((ethyl(4-fluorobenzyl)amino)methyl)-7,8-dimethylquinolin-4(1H)-one, was purchased from ChemDiv (San Diego, USA). Adenosine-5′-diphosphate monosodium salt, ATP disodium salt, UDP monosodium salt, and isoproterenol hydrochloride were purchased from Wako Pure Chemicals (Osaka, Japan). Uridine-5′-triphosphate was purchased from Sigma-Aldrich Japan (Tokyo, Japan). 2-Thio-UTP was purchased from Tocris Bioscience (Bristol, UK). Diadenosine 5′,5′″-P¹,P⁴- tetraphosphate was purchased from Jena Bioscience (Jena, Germany).

Sakuma K., Nakagawa H., Oikawa T., Noda M, & Ikeda S. (2017). Effects of 4(1H)-quinolinone derivative, a novel non-nucleotide allosteric purinergic P2Y2 agonist, on cardiomyocytes in neonatal rats. Scientific Reports, 7, 6050.

+ Open protocol

+ Expand

Murine Cortical Neuron Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neuronal cultures were prepared from E13.5 to E15.5 C57BL/6 WT or Ube3a^m−/p+ mice as previously described (Huang et al., 2012 ). Mouse cortices were dissected and trypsinized at 37°C for 10 min, and dissociated with a fire-polished Pasteur pipette in plating medium (Neurobasal medium with 5% fetal bovine serum, Glutamax, B27 (Life Technologies)), and Antibiotic-Antimycotic. Dissociated neurons were seeded onto 12 or 24-well plates with or without coverslips coated with poly-D-lysine (0.1 mg/ml) at a density of about 526 cells/mm². Cultured neurons were maintained at 37°C with 5% CO₂ and supplemented with Neurobasal medium containing 4.84 mg/ml Uridine 5′-triphosphate (Sigma), 2.46 mg/ml 5-fluoro 2-deoxyuridine (Sigma), Glutamax, B27, and 1× Antibiotic-Antimycotic at days in vitro (DIV) 3 and 9.

Yi J.J., Berrios J., Newbern J.M., Snider W.D., Philpot B.D., Hahn K.M, & Zylka M.J. (2015). An autism-linked mutation disables phosphorylation control of UBE3A. Cell, 162(4), 795-807.

+ Open protocol

+ Expand

Elucidating Purinergic Signaling Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols

Suramin and Reactive-Blue-2 (RB-2) were obtained from MP Biomedical (Santa Ana, CA, United States). DMEM/F12, advanced DMEM/F12, HEPES, L-glutamine, penicillin, streptomycin, FBS, Dulbecco’s PBS, apyrase, formyl-methionine-leucyl-phenylalanine (FMLP), LPSs, adenosine 5’-triphosphate (ATP), adenosine 5’-diphosphate (ADP), uridine 5’-triphosphate (UTP), uridine 5’-diphosphate (UDP), adenosine, Zm 241385, and fibroblast growth factor 2 (FGF2) were purchased from Sigma–Aldrich (Oakville, ON, Canada). Collagenase type I, SuperScript III, gentamicin, B-27 and N-2 supplements, polyinosinic–polycytidylic acid [poly(I:C)], EDTA, and TRIzol were obtained from Invitrogen (Carlsbad, CA, United States). Collagen type I was purchased from BD Bioscience (San Jose, CA, United States). Y-27632, mrEGF, Wnt-3a, and R-spondin were purchased from R&D Systems (Minneapolis, MN, United States). Noggin and M-CSF were purchased from PeproTech (QC, Canada). SYBR Green and DNAseI were from Roche Diagnostics (Indianapolis, IN, United States). Flagellin was obtained from InvivoGen (San Diego, CA, United States). Oligo(dt)18 was obtained from Fisher Scientific (Ottawa, ON, Canada). MRS 2500, MRS 2179, MRS 2578, AR-C 118925XX, PSB 1114, and 5-BDBD were purchased from Tocris Bioscience (Minneapolis, MN, United States).

Salem M., Tremblay A., Pelletier J., Robaye B, & Sévigny J. (2018). P2Y6 Receptors Regulate CXCL10 Expression and Secretion in Mouse Intestinal Epithelial Cells. Frontiers in Pharmacology, 9, 149.

+ Open protocol

+ Expand

Calcium Signaling Regulation in Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Krebs-Henseleit (KH) solution and the nominally Ca²⁺-free solution were prepared as previously described [24 (link)]. Membrane permeant acetoxymethyl (AM) ester forms of Fura-2 and Pluronic F127 were obtained from Invitrogen (Carlsbad, USA). Uridine 5′-triphosphate (UTP), uridine 5′-diphosphate (UDP), adenosine 5′-(γ-thio)triphosphate (ATPγS), forskolin, poly-l-arginine hydrochloride, SQ 22536, U73122, E₂, and G1 were obtained from Sigma-Aldrich (St. Louis, USA). G15 was obtained from Tocris (Bristol, UK). H89 dihydrochloride and MDL 12330A were obtained from Calbiochem (La Jolla, USA). All other general laboratory reagents were obtained from Sigma-Aldrich, and all cell culture reagents were obtained from Invitrogen.

Hao Y., Chow A.W., Yip W.C., Li C.H., Wan T.F., Tong B.C., Cheung K.H., Chan W.Y., Chen Y., Cheng C.H, & Ko W.H. (2016). G protein-coupled estrogen receptor inhibits the P2Y receptor-mediated Ca2+ signaling pathway in human airway epithelia. Pflugers Archiv, 468, 1489-1503.

+ Open protocol

+ Expand

Neonatal Rat Cardiomyocyte Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adenosine 5′-triphosphate (ATP), guanosine 5′-triphosphate (GTP), uridine 5′-triphosphate (UTP), cytidine 5′-triphosphate (CTP), and oligomycin were purchased from Sigma-Aldrich (St Louis, MO, USA). Imaging compatible Nunc™ Lab-Tek™ II 8-well chamber slides (Cat#: 155409PK), Hoechst 33,342 (Cat#: H3570), Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, Cat#: T34075), neonatal rat cardiomyocyte isolation enzymes, supplements, and primary cell isolation medias were purchased form Thermo Fisher Scientific (Waltham, MA). Neonatal rat hearts were generously donated from FIU’s animal facility. All the other chemicals were of analytical reagent grade and were used as received without further purification.

Liu W., Zhu X., Mozneb M., Nagahara L., Hu T.Y, & Li C.Z. (2021). Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe. Mikrochimica Acta, 188(10), 352.

+ Open protocol

+ Expand

Quantification of Nucleotide Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were of analytical grade. Bovine serum albumin (BSA), malachite green, adenosine 5′ -triphosphate (ATP), adenosine 5′ -diphosphate (ADP), cytidine 5′-triphosphate disodium salt (CTP), adenosine 5′ -monophosphate (AMP), uridine 5’-triphosphate (UTP), uridine 5’-diphosphate (UDP), guanosine-5’-triphosphate (GTP), phosphate-buffered saline (DPBS), 4-(2-hydroxyethyl)-1-piperazineetahnesulfonic acid (HEPES), ammonium molybdate, Triton X-100, phenylmethylsulphonyl fluoride (PMSF), pyruvate kinase, phosphoenol-pyruvate (PEP), luciferase, coenzyme A and P1,P5-Di (adenosine-5´) pentaphosphate pentasodium salt (Ap5A) were purchased from Sigma-Aldrich (St Louis, MO, USA). D-luciferin was purchased from Molecular Probes Inc. (Eugene, OR, USA).

Saffioti N.A., Alvarez C.L., Bazzi Z., Gentilini M.V., Gondolesi G.E., Schwarzbaum P.J, & Schachter J. (2023). Dynamic recycling of extracellular ATP in human epithelial intestinal cells. PLOS Computational Biology, 19(6), e1011196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!