Colo320

Colorectal cancer cell lines (colo205, HCT116, HT29, SW480, and DLD1) were obtained from ATCC and colo320 was provided by Riken BRC through the National BioResource Project, Japan. The detail of cell culture and chemicals are shown in Document S1.

The CRC cell lines used in this study were selected on the basis of DUSP4 expression data from the Cancer Cell Line Encyclopedia (https://portals.broadinstitute.org/ccle). Five CRC cell lines, COLO‐320, SNU‐283, CW‐2, SNU‐503, and SNU‐1033, express low levels of DUSP4, and three cell lines, SNU‐C2A, SNU‐C5, and HCT 116, express high levels of DUSP4. The CRC cell lines COLO‐320, CW‐2, and HCT 116 were obtained from RIKEN BioResource Center (Ibaraki, Japan), and SNU‐283, SNU‐503, SNU‐1033, SNU‐C2A, and SNU‐C5 were obtained from the Korean Cell Line Bank (Seoul, Korea). The human colonic epithelial cells (HCoEpiC) were purchased from ScienCell (Carlsbad, CA, USA). The human glioblastoma cell line A‐172, in which the promoter region of DUSP4 is known to be hypermethylated,9 was obtained from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Miyagi, Japan). All of the cell lines were cultured in accordance with the suppliers' instructions. The genotypes of EGFR, KRAS, HRAS, NRAS, BRAF, APC, CTNNB1, TP53 and CDKN2A genes in all eight CRC cell lines were obtained from the Cancer Cell Line Encyclopedia. All of them except for HRAS, NRAS, and CDKN2A are summarized in Table 1, because these three genes are not mutated in any of the cell lines.

The human CRC cell lines were obtained since July 2012. DLD1 was obtained from Institute of Development, Aging and Cancer, Cell Resource Center for Biomedical Research, Tohoku University which was checked by short tandem repeat PCR (STR-PCR). SW620 was purchased from European Collection of Cell Cultures. HCT116 were purchased from American Tissue Culture Collection. LoVo and colo320 were purchased from Riken BioResource Center CELL BANK. HT29 was kindly provided from Division of Gene Regulation, Institute of Advanced Medical Research, School of medicine, Keio University and checked by STR-PCR. The cells were cultured in the recommended medium supplemented with 10% fetal bovine serum (Gibco-BRL, CA, USA) at 37°C in a humidified atmosphere of 5% CO₂ to 95% air. The gene profile of the cell lines is summarized in Supporting Information Table 1.