Eosin y

Manufactured by Avantor

Sourced in Germany, United States, Denmark

Eosin Y is a synthetic dye commonly used in various laboratory applications. It is a fluorescent, water-soluble dye that exhibits a pink-red color. Eosin Y is a commonly used counterstain in histological and cytological staining procedures, particularly in combination with hematoxylin.

Automatically generated - may contain errors

Lab products found in correlation

Bx40 microscope, by Olympus (7 mentions) Hematoxylin qs, by Vector Laboratories (7 mentions) Positively charged slides, by Avantor (7 mentions) Dp25 imaging system, by Olympus (6 mentions) Cytoseal xyl mounting media, by Thermo Fisher Scientific (4 mentions) Vectamount mounting media, by Vector Laboratories (3 mentions) Fluorometric activity assay, by Merck Group (2 mentions) Total bilirubin elisa, by Cusabio (2 mentions) Catalyst one serum chemistry analyzer, by IDEXX (2 mentions) Harris haematoxylin, by Thermo Fisher Scientific (2 mentions) 2 5 dihydroxyacetophenone, by Merck Group (2 mentions) Chloroform, by Merck Group (2 mentions) Hematoxylin, by Merck Group (2 mentions) Xylene ar grade, by Biosolve (2 mentions) Image pro plus 7, by Media Cybernetics (1 mentions) Absolute ethanol, by Avantor (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Toluidine blue, by Merck Group (1 mentions) Periodic acid schiff, by Merck Group (1 mentions) Xylene, by Avantor (1 mentions)

17 protocols using eosin y

Histological and Biochemical Analysis of Liver in AOM-Treated Mice

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Paraffin-embedded livers from vehicle and AOM-treated mice were sectioned into 3 µm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for one minute followed by staining for one minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum ALT and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorometric activity assay (Sigma-Aldrich, St. Louis, MO). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to the manufacturer’s instructions.

McMillin M., Frampton G., Tobin R., Dusio G., Smith J., Shin H., Newell-Rogers M.K., Grant S, & DeMorrow S. (2015). TGR5 signaling reduces neuroinflammation during hepatic encephalopathy. Journal of neurochemistry, 135(3), 565-576.

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Histological Analysis of Liver Tissue

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Paraffin-embedded livers were cut into 4 μm sections and mounted onto positively charged slides (VWR, Radnor, PA, USA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA, USA) for 1 min followed by staining for 1 min with eosin Y (Amresco, Solon, OH, USA) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA, USA). Serum alanine aminotransferase (ALT) concentrations were measured using a commercially available kit from Sigma-Aldrich with all assays and subsequent analyses performed according to the instructions provided by the manufacturer.

McMillin M., Frampton G., Grant S., Khan S., Diocares J., Petrescu A., Wyatt A., Kain J., Jefferson B, & DeMorrow S. (2017). Bile Acid-Mediated Sphingosine-1-Phosphate Receptor 2 Signaling Promotes Neuroinflammation during Hepatic Encephalopathy in Mice. Frontiers in Cellular Neuroscience, 11, 191.

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Histological Analysis of Paraffin-Embedded Liver Sections

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Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA). All images are taken as × 200 magnification.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA).

McMillin M., Grant S., Frampton G., Petrescu A.D., Williams E., Jefferson B., Thomas A., Brahmaroutu A, & DeMorrow S. (2019). Elevated circulating TGFβ1 during acute liver failure activates TGFβR2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice. Journal of Neuroinflammation, 16, 69.

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Histological Assessment of Liver Damage

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Liver damage was assessed by H&E staining according to previously published protocols.⁷ (link) Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories) for 1 minute followed by staining for 1 minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides then were dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (Thermo Fisher Scientific, Waltham, MA). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Center Valley, PA).
Liver function was assessed by measuring plasma alanine aminotransferase and aspartate aminotransferase using the IDEXX Catalyst One machine from IDEXX Laboratories, Inc.

McMillin M., Grant S., Frampton G., Petrescu A.D., Kain J., Williams E., Haines R., Canady L, & DeMorrow S. (2018). FXR-Mediated Cortical Cholesterol Accumulation Contributes to the Pathogenesis of Type A Hepatic Encephalopathy. Cellular and Molecular Gastroenterology and Hepatology, 6(1), 47-63.

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Histological Liver Analysis and Serum Biomarkers

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Paraffin-embedded livers were cut into 3-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for 1 min, followed by staining for 1 min with eosin Y (Amresco, Solon, OH), and rinsed in 95 % ethanol. The slides were then dipped into 100 % ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum alanine aminotransferasase (ALT) and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorimetric activity assay. Total bilirubin was assayed using a total bilirubin ELISA. All assays and subsequent analyses were performed according to the instructions provided by the manufacturers.

McMillin M., Grant S., Frampton G., Andry S., Brown A, & DeMorrow S. (2016). Fractalkine suppression during hepatic encephalopathy promotes neuroinflammation in mice. Journal of Neuroinflammation, 13(1), 198.

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Histochemical and Biochemical Analysis of Liver Function

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Paraffin-embedded livers were sectioned into 3 μm sections and mounted onto positively charged slides (VWR, Radnor, PA, USA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA, USA) for 1 min followed by staining for 1 min with eosin Y (Amresco, Solon, OH, USA) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA, USA).
Serum alanine aminotransferase (ALT) and bilirubin were assessed using commercially available kits. ALT measurement was performed using a fluorometric activity assay (Sigma-Aldrich). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to manufacturers’ instructions.

McMillin M., Frampton G., Thompson M., Galindo C., Standeford H., Whittington E., Alpini G, & DeMorrow S. (2014). Neuronal CCL2 is upregulated during hepatic encephalopathy and contributes to microglia activation and neurological decline. Journal of Neuroinflammation, 11, 121.

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Quantification of Liver Necrosis and Function

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Paraffin-embedded livers were cut into 4 μm sections and mounted onto positively-charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with Eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher Scientific). The slides were viewed and imaged using an Olympus BX40 microscope with a DP25 imaging system (Olympus, Center Valley, PA). Percentage area of necrosis was calculated using 20× field views, drawing areas devoid of hepatocyte nuclei and calculating area using ImageJ software (National Institutes of Health, Bethesda, MD). In each mouse, the necrosis from 5 to 10 fields were quantified and averaged together to determine necrotic area.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA). For control groups, serum was diluted 1:2 in deionized H₂0 and for APAP-treated groups, serum was diluted 1:9 in deionized H₂0 prior to running on the analyzer.

Frampton G., Reddy P., Jefferson B., Ali M., Khan D, & McMillin M. (2020). Inhibition of thrombospondin-1 reduces glutathione activity and worsens acute liver injury during acetaminophen hepatotoxicity in mice. Toxicology and applied pharmacology, 409, 115323.

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Histological Analysis of Intestinal Tissues

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Fixated tissues were dehydrated in an ethanol gradient of 77%‐99% (absolute ethanol, cat. no. 83813.360, VWR), cleared using xylene (cat. no. 28973.363, VWR) and embedded in paraffin (cat. no. 2270.60.60, Hounisen, Skanderborg, Denmark). Sections of 2 μm were stained with Meyer's Hematoxylin (cat. no. AMPQ00254.0500, Ampliqon, Odense, Denmark) and Eosin Y (cat. no. 341973R, VWR) to identify eosinophils and 0.5% Toluidine Blue (TB; cat. no. 89640, Sigma‐Aldrich) in 1 M hydrochloric acid to identify mast cells, or Periodic acid‐Schiff (PAS; periodic acid: Cat. no. 1.00524.0025, Merck and Schiff′s reagent: Cat. 3952016, Sigma‐Aldrich) to identify goblet cells.³⁴ Slides were examined using a Leica DMR upright microscope (Leica Microsystems GmbH, Wetzlar, Germany). The software ImagePro Plus 7.0 (MediaCybernetics, Rockville, MD, US) was used for image analysis. Villus length was measured from the villus tip to the crypt‐villus junction. Cell count and villi length in the SI were averaged from three sections of three similar consecutive villi and crypts. Cell count in the colon was averaged from six individual crypts. Analysis of histological sections was performed blinded.

Locke A.V., Larsen J.M., Graversen K.B., Licht T.R., Bahl M.I, & Bøgh K.L. (2022). Amoxicillin does not affect the development of cow’s milk allergy in a Brown Norway rat model. Scandinavian Journal of Immunology, 95(5), e13148.

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Histological Analysis of Tumor Tissue

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Formalin fixed paraffin embedded tumor tissue from mice treated for 2 days were sliced manually with a manual microtome (Leica, RM2155) to 5 μm depth. H&E staining was preformed using Gill's II Hematoxylin (Sigma-Aldrich, GHS216) and Eosin Y (VWR, 95057-848). For IHC, slides were stained with cleaved caspase 3 antibody (Cell Signaling Technology, 9661), and counterstained with Gill's II Hematoxylin. Slides were visualized using a Leica DMi8 automated microscope and also scanned by HistoWiz for low-power visualization.

Yi J.S., Sias-Garcia O., Nasholm N., Hu X., Iniguez A.B., Hall M.D., Davis M., Guha R., Moreno-Smith M., Barbieri E., Duong K., Koach J., Qi J., Bradner J.E., Stegmaier K., Weiss W.A, & Gustafson W.C. (2021). The synergy of BET inhibitors with aurora A kinase inhibitors in MYCN-amplified neuroblastoma is heightened with functional TP53. Neoplasia (New York, N.Y.), 23(6), 624-633.

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Histological Evaluation of Nerve Tissue

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Haematoxylin & eosin (H&E; Raymond A Lamb Ltd., U.K.) staining of native and acellular nerve segments was used to evaluate tissue histoarchitecture. Samples were immersed in Harris haematoxylin (Thermo Fisher Scientific Ltd., U.K) (1 min) and rinsed under tap water for blueing (3 min). Slides were then immersed into eosin Y (VWR International) (3 min), dehydrated, cleared and mounted using DPX mountant before being viewed under Kohler illumination.

Zilic L., Wilshaw S, & Haycock J.W. (2016). Decellularisation and histological characterisation of porcine peripheral nerves. Biotechnology and Bioengineering, 113(9), 2041-2053.

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