C57BL/6, Rag2-/-, Tbx21-/-, CD11c-Cre, Irf8flox/flox, and Tbx21flox/flox mice were obtained from Jackson Laboratory (Bar Harbor, ME) and Rag2-/-γc-/- mice were obtained from Taconic (Rensselaer, NY). All control and experimental mice were age- and sex-matched within all individual experiments. This study included both male and female mice, and the data derived from male and female mice identified no sex-specific differences in the performed experiments.
Rag2 γc mice
Manufactured by Taconic Biosciences
Sourced in Montenegro, United States
Rag2−/−γc−/− mice are a genetically-engineered mouse model with a targeted deletion of the Rag2 and gamma chain (γc) genes. These mice exhibit a severe combined immunodeficiency (SCID) phenotype, characterized by the lack of functional T cells and B cells.
Automatically generated - may contain errors
Lab products found in correlation
Human ab serum, by Merck Group (3 mentions)
Irf8flox flox, by Jackson ImmunoResearch (2 mentions)
Tbx21flox flox, by Jackson ImmunoResearch (2 mentions)
Cd11c cre, by Jackson ImmunoResearch (2 mentions)
C57bl 6, by Jackson ImmunoResearch (2 mentions)
Cd4 cre female mice, by Taconic Biosciences (2 mentions)
C57bl 6 and 2d2 mice, by Jackson ImmunoResearch (2 mentions)
Rag2 mice, by Taconic Biosciences (2 mentions)
Rag1 mice, by Jackson ImmunoResearch (2 mentions)
Tbx21, by Jackson ImmunoResearch (1 mentions)
C57bl 6 b6 mice, by Taconic Biosciences (1 mentions)
Magnetic bead negative selection kit, by Miltenyi Biotec (1 mentions)
C57bl 6j wt, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Taconic Biosciences (1 mentions)
Cd1d mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
Casp1, by Jackson ImmunoResearch (1 mentions)
Tbx21 cre, by Jackson ImmunoResearch (1 mentions)
R26r eyfp, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Koatech (1 mentions)
19 protocols using rag2 γc mice
1
Mouse Strains for Immunological Studies
2
Humanized Mouse Model for GVHD Research
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Example 77
Blood from healthy human donors is collected in a tube containing sodium heparin. The blood is diluted in an equal volume of Ca2+ and Mg2+-free phosphate buffered saline with 2% v/v fetal bovine serum and centrifuged at room temperate at 200×g for 10 minutes. The white “huffy coat” layer is removed to yield human peripheral mononuclear cells (huPBMCs), washed five times in RPMI 1640, and diluted (2×106 cells/mL) in RPMI 1640 with &complemented 20% human AB serum (56° C., 30 minutes, Sigma) and 150 μg/mL gentamicin.
At 4- to 5 weeks old, the Rag2−/−γc−/− mice (purchased from Taconic) are pretreated with liposome-clodronate (VU Medisch Centrum) and sublethally irradiated (1 Gy/6 g), then transplanted intraperitoneally with 3.0×107 huPBMCs. After 4 weeks, these humanized mice sublethally irradiated and, one day later, are injected intravenously with 1.0×1.07 allogeneic huPBMCs (1 Gy/6 g). The transplanted mice are monitored daily for GVHD symptoms including weight loss, temperature changes, and diarrhea. (Zheng J, Liu Y, Liu U, Liu M, Xiang Z, Lam K-T, Lewis D B, Lau Y-L, Tu W, 2013. Human CD8+ Regulatory T Cells Inhibit GVHD and Preserve General Immunity in Humanized Mice Sci. Transl Med 5:168ra9.)
3
Characterization of Arf Null Mice
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Arf−/− (Kamijo et al., 1997 (link))and Arfgf/gfp mice (Sherr, 2004 (link)) are both p19Arf null strains, were all on a backcrossed C57BL/6J background and were provided by C. Sherr (St. Jude Children’s Research Hospital, Memphis, TN). They were used between 8 and 12 wk of age. The experiments in Figs. 1 and 2 used the Arfgf/gfp mice, all the other experiments were done using Arf−/− mice. Female C57BL/6J and Rag2−/− γc−/− mice (Taconic) were used as transplantation recipients. Transgenic mice on a B6 background carrying an enhanced GFP gene whose expression is controlled by the proximal lck promoter (plck-GFP mice) were a gift from T. Nakayama (Chiba University, Chiba, Japan).
All experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital.
All experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital.
4
Murine Models for Neurological Research
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Male C57BL/6 (B6) mice and Rag2−/−γc−/− mice were purchased from Taconic (Taconic Biosciences). The mutant mice were back-crossed to the B6 background for 12 generations. For all experiments, 6 - to 12-week-old, 23–25 g body weight, age-matched littermates were used. All mice were randomly assigned to experimental groups. Randomization was based on the random number generator function in Microsoft Excel 2013. Mice were housed under standardized light-dark cycle conditions with access to food and water ad libitum. All surgeries were performed under isoflurane anesthesia. Mice were housed in pathogen-free conditions at the animal facilities. All animal experiments were performed in accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and in accordance with the ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines. Animal studies were approved by the Animal Care and Use Committees of the Barrow Neurological Institute and Tianjin Neurological Institute.
5
Conditional Deletion of Jmjd3 in Mice
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Jmjd3flox/flox (Jmjd3f/f) mice were generated by targeting exons 15-21 (encoding the JmjC-catalytic domain) using a Cre-LoxP system. Eight-week-old Jmjd3f/f male mice were crossed with eight-week-old CD4-Cre female mice (Taconic) to generate Jmjd3 cKO mice. C57BL/6 and 2D2 mice were obtained from the Jackson Laboratory. Rag2−/−γc−/− mice were purchased from Taconic. Foxp3-GFP reporter mice were kindly provided by Dr. Alexander Rudensky (Memorial Sloan-Kettering Cancer Center, NY). All mice were rederived by standard embryo transfer and maintained in pathogen-free animal facilities at Baylor College of Medicine and the Houston Methodist Research Institute. This study was reviewed and approved by Institutional Animal Care and Use Committee at Baylor College of Medicine and the Houston Methodist Research Institute.
6
Conditional Deletion of Jmjd3 in Mice
7
Isolation and Characterization of Murine NK Cells
8
Genetically Engineered Mouse Models
9
Humanized Mouse Model of GVHD
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Example 77
Blood from healthy human donors is collected in a tube containing sodium heparin. The blood is diluted in an equal volume of Ca2+- and Mg2+-free phosphate buffered saline with 2% v/v fetal bovine serum and centrifuged at room temperate at 200× g for 10 minutes. The white “buffy coat” layer is removed to yield human peripheral mononuclear cells (huPBMCs), washed five times in RPMI 1640, and diluted (2×106 cells/mL) in RPMI 1640 with decomplemented 20% human AB serum (56° C., 30 minutes, Sigma) and 150 μg/mL gentamicin.
At 4- to 5 weeks old, the Rag2−/−γc−/− mice (purchased from Taconic) are pretreated with liposome-clodronate Medisch Centrum) and sublethally irradiated (1 Cay/6 g), then transplanted intraperitoneally with 3.0×10′ huPBMCs. After 4 weeks, these humanized mice sublethally irradiated and, one day later, are injected intravenously with 1.0×107 allogeneic huPBMCs (1 Cy/6 g). The transplanted mice are monitored daily for GVHD symptoms including weight loss, temperature changes, and diarrhea. (Meng J, Liu Y, Liu U Liu M, Xiang Z, Lam K-T, Lewis D B, Lau Y-L, Tu W, 2013. Human CD8+ Regulatory T Cells Inhibit GVHD and Preserve General Immunity in Humanized Mice Sci Transl Med 5:168ra9.)
10
Mice for Immunology Research
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10–12-wk-old male mice were used in this study. C57BL/6 mice were purchased from Charles River Laboratories. Rag2−/−γc−/− mice were purchased from Taconic. CD1d−/− mice were purchased from the Jackson Laboratory. All mutant mice were backcrossed to the C57BL/6 background for 8–12 generations. Mice were housed no more than five animals per cage in pathogen-free conditions under a standardized light–dark cycle with free access to food and water. For all experiments, age-matched male littermates were used between experimental groups. Animal surgeries were performed under anesthesia. All animal experiments were approved by the Committee on the Ethics of Animal Experiments of Tianjin Neurological Institute (Tianjin, China). All experiments were conducted in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and were designed and performed according to the Animal Research: Reporting In Vivo Experiments guidelines (https://arriveguidelines.org/ ).
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