SC (purity of 97.8%) was synthesized and supplied by The Structure-Activity Laboratory on Department of Chemistry of Federal University of Santa Catarina, Brazil. Soy lecithin (Lipoid S-75®) and medium chain triglycerides (MCT) were kindly given by Lipoid GmbH (Ludwigshafen, Germany). Polysorbates 80 and 20 (Tween 80® and Tween 20®, respectively), sorbitan monooleate (Span 80®), and Nile red were purchased from Sigma Chemical Company (St Louis, MO, USA). Trifluoroacetic acid was purchased from Merck (Deisenhofen, Germany). Ultrapure water was obtained from a Milli-Q® Plus apparatus by Millipore (Merck Millipore, Billerica, MA, USA). Methanol liquid chromatography (LC) grade was obtained from Tedia (Rio de Janeiro, Brazil). All other reagents were of analytical grade.
Sorbitan monooleate span 80
Manufactured by Merck Group
Sourced in United States, Germany
Sorbitan monooleate, also known as Span 80, is a non-ionic surfactant. It is primarily used as an emulsifier, wetting agent, and dispersing agent in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyoxyethylenesorbitan monooleate tween 80, by Merck Group (3 mentions)
Nile red, by Merck Group (2 mentions)
Chloroauric acid haucl4, by Merck Group (2 mentions)
Polyethylene glycol sorbitan monooleate tween 80, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Chloroform, by Merck Group (2 mentions)
Poloxamer 407 pluronic f127, by Merck Group (2 mentions)
Tween 20, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Milli q plus apparatus, by Merck Group (1 mentions)
Phosphate buffered saline ph 7.4, by Merck Group (1 mentions)
Dimethylaminopyridine, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Methacrylic acid, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
27 protocols using sorbitan monooleate span 80
1
Synthesis and Characterization of SC Nanoformulation
2
Formulation and Characterization of Ibuprofen Emulsion
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Polysorbate 80 (Tween 80®) was purchased from Uniqema (Everberg, Belgium). Ibuprofen was kindly donated from Medinfar (Amadora, Portugal). Polyvinylpyrrolidone (PVP) K30 was purchased from Fluka (Switzerland). Phosphate buffered saline, pH 7.4 and sorbitan monooleate (Span 80®) were obtained from Sigma-Aldrich (Steinheim, Germany). Ultra-purified water was obtained from Milli® Q Plus system, home supplied.
3
Synthesis of Gold Nanoparticles
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Lavender oil (Lavandula angustifolia) (CAS number 8000-28-0), chloroauric acid (HAuCl4), polyethylene glycol sorbitan monooleate (Tween 80) and sorbitan monooleate (Span 80) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Synthesis and Characterization of Resveratrol Derivatives
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The solvents used: acetone, ethanol, methanol, n-hexane, chloroform, 2-propanol, and ethyl ether were purchased from Carlo Erba Reagenti (Milan, Italy). Chloroform was purified by standard procedures. Resveratrol or 3,5,4’-trihydroxy-trans-stilbene (MM = 22,825 g/mol), D-Mannose (MM = 180,156 g/mol), ciprofloxacin (MM = 331,346), methacrylic acid (MAA), 85% formic acid (FA), 85% phosphoric acid (AP), sodium nitrite (SN), dicyclohexylcarbodimide (DCC), dimethylaminopyridine (DMAP), dimethyl sulfoxide (DMSO), potassium chloride (KCl), hydrochloric acid (HCl), sodium phosphate (Na3PO4), citric acid, disodium hydrogen phosphate (Na2HPO4), sorbitan monooleate (Span 80) and polyoxyethylene sorbitan monooleate (Tween 80), N,N′-ethylene bis-acrylamide (EBA) were purchased from Sigma-Aldrich (Sigma Chemical CO, St. Louis, MO, USA).
5
Letrozole-Loaded Nanoparticle Formulations
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Letrozole was kindly donated by Tofigh Daru (Tehran, Iran). Sorbitan monolaurate (Span20, Cat# S6635), Sorbitan monostearate (Span60, Cat# S7710), Sorbitan monooleate (Span80, Cat# S6760), Cholesterol (CHOL, Cat# C8667), dicetylphosphate (DCP, Cat# 850042P), Sodium dodecyl sulfate (SDS, Cat# L3771) and dialysis membrane (MWCO 12kDa, Cat# D6191), Nile red and Coumarin 6 were obtained from Sigma Aldrich (USA). Dimethyl sulfoxide (DMSO, Cat# 109678), chloroform (Cat# 107024), Amicon (Ultra-15-Membrane, MWCO 30kDa, Cat# C82301) were purchased from Merck (Germany). Trypsin-EDTA (Cat# 15400054), Trypan blue (Cat# 15250061), RPMI-1640 culture medium, DMEM (Dulbecco’s Modified Eagle culture medium, Cat# 11965092), phosphate-buffered saline (PBS, Cat# 10010049), fetal bovine serum (FBS, Cat# 16000044), MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Cat# M6494) and penicillin/streptomycin (PS, Cat# 10378016) 100 X were purchased from Gibco (USA). The MDA-MB-231, MCF-7, and MCF10A cell lines were obtained from Pasteur Cell Bank (Iran). The Annexin V-FITC flow cytometric kit was obtained from Affymetrix Biosciences (USA). RNA extraction and cDNA synthesis kits were purchased from Transgene Biotech, China (Cat#. ER101-01 and AE301-02).
6
Lipid-based Nanocarrier Formulation
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Hydrogenated palm oil, (Softisan 154 or S154) a gift from Condea (Witten, Germany), and stearic acid (SA, Sigma Aldrich, USA) were the lipids used in this study. Water soluble surfactants used were polioxiethylene-20-sorbitan monooleate (Tween 80- Sigma Aldrich, USA), Poloxamer407 (Pluronic F127, Sigma Aldrich, USA). Furthermore, lipo-soluble surfactants used were Sorbitanmonooleate (Span 80, Sigma Aldrich, USA), and soy lecithin (Sigma-Aldrich). Doxycycline hyclate (Sigma-Aldrich). Water used in our study was distilled twice and purified by an appropriate filter.
7
Synthesis and Characterization of 4-Nitroaniline
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Kerosene and sorbitan monooleate (Span 80) were supplied by Sigma Aldrich, Steinheim (Germany). 4-Nitroaniline (98%) and HCl (37%) were obtained from Panreac, Darmstadt (Germany). All chemicals were used without any further purification. Deionized water was used for making all the aqueous solutions.
8
Antioxidant Capacity Evaluation Protocol
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Folin-Ciocalteu reagent, sodium alginate (low viscosity), sorbitan monooleate (span 80), calcium chloride, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Trolox, ferrous chloride, 2,4,6-tripyridyl-s-triazine (TPTZ) and sodium acetate tetrahydrate were purchased from Sigma-Aldrich Chemical Company (USA). All other reagents were selected in analytical grade.
9
Olive Mill Wastewater Characterization and Antioxidant Potential
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Olive mill wastewater (OMW) used in this study was obtained by means of a three-phase olive oil extraction and centrifugation system and was kindly provided by Mamalan Agro Industrial Company (Zanjan, Iran). Miglyol 812 oil, a mixture of C6-C12 medium chain triglycerides (MCT), was supplied by Sasol GmbH (Hamburg, Germany). Folin-Ciocalteu reagent and hydrochloric acid (37%) were purchased from VWR International Eurolab (Llinars del Vallès, Spain) and Acros Organics (Geel, Belgium), respectively. Sodium carbonate, methanol, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), sorbitan monooleate (Span 80) and polyoxyethylene (20) sorbitan monooleate (Tween 80) were purchased from Sigma-Aldrich (Darmstadt, Germany). Milli-Q water (Millipore, St. Louis, MO, USA) was used in all samples.
10
Evaluating E. globulus Oil Efficacy
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E. globulus oil was supplied by Ferquima (São Paulo, Brazil). Sorbitan monooleate (Span 80®) was supplied by Sigma-Aldrich (São Paulo, Brazil). Polysorbate 80 (Tween 80®) was supplied by LabSynth® (São Paulo, Brazil). Caprylic/capric triglyceride mixture was acquired from Alpha Química Ltda. (Porto Alegre, Brazil). Histopaque was supplied by Sigma-Aldrich (São Paulo, Brazil). Fetal Bovine Serum, RPMI 1640, and Penicillin/Streptomycin were supplied by Cultilab (São Paulo, Brazil). Dimethyl sulfoxide (DMSO) and trypan blue were supplied by Nuclear (São Paulo, Brazil).
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E. globulus oil was supplied by Ferquima (São Paulo, Brazil). Sorbitan monooleate (Span 80®) was supplied by Sigma-Aldrich (São Paulo, Brazil). Polysorbate 80 (Tween 80®) was supplied by LabSynth® (São Paulo, Brazil). Caprylic/capric triglyceride mixture was acquired from Alpha Química Ltda. (Porto Alegre, Brazil). Histopaque was supplied by Sigma-Aldrich (São Paulo, Brazil). Fetal Bovine Serum, RPMI 1640, and Penicillin/Streptomycin were supplied by Cultilab (São Paulo, Brazil). Dimethyl sulfoxide (DMSO) and trypan blue were supplied by Nuclear (São Paulo, Brazil).
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