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Nanolc 2d hplc nanoflow system

Manufactured by AB Sciex

The NanoLC-2D HPLC nanoflow system is a high-performance liquid chromatography (HPLC) instrument designed for nanoflow applications. It is capable of delivering precise and reproducible flow rates in the nanoliter per minute range, making it suitable for applications that require high sensitivity and low sample volumes, such as proteomics and metabolomics research.

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2 protocols using nanolc 2d hplc nanoflow system

1

Peptide Analysis by LTQ Orbitrap XL MS

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The peptide samples were analyzed on an LTQ Orbitrap XL mass spectrometer (Thermo Scientific) using Xcalibur version 2.0.7 coupled to an Eksigent NanoLC-2D HPLC nanoflow system (dual pump system with one analytical column; Eksigent) via a nanoelectrospray ion source using a liquid junction (Thermo Scientific), as described previously 29 . The raw data were converted to mzXML format and searched using Sorcerer against a target-decoy Swissprot mouse protein database. False discovery rate was controlled below 1% using Trans Proteomic Pipeline (TPP, v 4.6.1).
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2

Peptide Analysis by LTQ Orbitrap XL MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
The peptide samples were analyzed on an LTQ Orbitrap XL mass spectrometer (Thermo Scientific) using Xcalibur version 2.0.7 coupled to an Eksigent NanoLC-2D HPLC nanoflow system (dual pump system with one analytical column; Eksigent) via a nanoelectrospray ion source using a liquid junction (Thermo Scientific), as described previously 29 . The raw data were converted to mzXML format and searched using Sorcerer against a target-decoy Swissprot mouse protein database. False discovery rate was controlled below 1% using Trans Proteomic Pipeline (TPP, v 4.6.1).
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