Charcoal stripped fbs css

Manufactured by Thermo Fisher Scientific

Charcoal stripped FBS (CSS) is a specialized serum product used in cell culture applications. It is prepared by treating fetal bovine serum (FBS) with activated charcoal to remove various components, including steroid hormones, growth factors, and other small molecules. This process results in a serum that is depleted of these substances, making it a useful tool for researchers studying the effects of these components on cell behavior and signaling pathways.

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Lab products found in correlation

Adenine hemisulfate, by Merck Group (1 mentions) Tissue culture plasticware, by Thermo Fisher Scientific (1 mentions) Insulin solution, by Merck Group (1 mentions) Dmem low glucose media, by Thermo Fisher Scientific (1 mentions) Dmem high glucose media, by Thermo Fisher Scientific (1 mentions) Ham s f12 media, by Thermo Fisher Scientific (1 mentions) Nuclei ez prep kit, by Merck Group (1 mentions) Zapoglobin, by Beckman Coulter (1 mentions) Isoton 2, by Beckman Coulter (1 mentions) Horseradish peroxidase conjugated donkey anti rabbit and sheep anti mouse antibodies, by GE Healthcare (1 mentions) Apo transferrin, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) D biotin, by Merck Group (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Lncap prostate cancer cell line, by American Type Culture Collection (1 mentions)

2 protocols using charcoal stripped fbs css

Cell Culture Media and Reagents Procurement

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DMEM low glucose media, DMEM high glucose media, Hams’ F‐12 media, DMEM/F‐12 media (1:1), penicillin/streptomycin solution and phosphate buffered saline (PBS) were purchased from Invitrogen (Carlsbad, CA). The media additives d‐biotin, adenine hemisulfate, insulin solution, apo‐transferrin, and Nuclei EZ Prep kit were purchased from Sigma–Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from HyClone (Logan, UT). Charcoal stripped FBS (CSS) was prepared within our laboratory or purchased from Invitrogen (Carlsbad, CA). Zapoglobin and Isoton II were purchased from Beckman Coulter Inc. (Fullerton, CA). Rabbit anti‐mouse IgG secondary antibody was obtained from Zymed Laboratories, Inc. Both horseradish peroxidase‐conjugated donkey anti‐rabbit and sheep anti‐mouse antibodies were purchased from GE Healthcare Biosciences (Pittsburg, PA). All tissue culture plasticware and additional chemicals were purchased from Fisher Scientific (Suwanee, GA).

Olokpa E., Bolden A, & Stewart L.V. (2016). The Androgen Receptor Regulates PPARγ Expression and Activity in Human Prostate Cancer Cells. Journal of Cellular Physiology, 231(12), 2664-2672.

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Unique Salivary Gland Tumor Cell Line

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The RET981 developed by our group, is the only cell line, currently available in the field. RET981 was derived from a female patient with metastatic poorly differentiated, mixed malignant tumor of salivary gland (38 (link)). We tested the STR analysis and indicated the unique profile without any contamination (Supplementary Figure 1). RET981 and LNCaP prostate cancer cell line (ATCC) were maintained in RPMI 1640 medium with 10% FBS. A253 salivary epidermoid carcinoma cell (ATCC) and VCaP prostate cancer cell line (ATCC) were cultured in DMEM medium with 10% FBS. For androgen treatment, cells were cultured in phenol red-free medium supplemented with 10% charcoal-stripped FBS (CSS) (Invitrogen) for 24 hours and then treated with or without 1nM DHT.

Mitani Y., Rao P.H., Maity S.N., Lee Y.C., Ferrarotto R., Post J.C., Licitra L., Lippman S.M., Kies M.S., Weber R.S., Caulin C., Lin S.H, & El-Naggar A.K. (2014). Alterations Associated with Androgen Receptor Gene Activation in Salivary Duct Carcinoma (SDC) of Both Sexes: Potential Therapeutic Ramifications. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(24), 6570-6581.

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