Feeder-free iPSCs were dissociated with TryplE and seeded onto AggreWell™800 plate (10,000 cells per EB; Stem Cell Technologies) in E8 media supplemented with 10 μM ROCK inhibitor Y27632 for the first 24 h; we changed 75% of the media daily with STEMdiff™ Neural Induction Medium (NIM, Stem Cell Technologies). EBs were harvested after 5 days and plated onto poly-L-ornithine/laminin (PLO/L, Sigma) coated plates. 1–2 day(s) after attachment, prominent neural rosette structures were visible inside the attached neural aggregates. Rosettes were formed by cells expressing proteins characteristic of progenitor marker of PAX6, as described in Supplementary Information .
Plo l
Manufactured by Merck Group
The PLO/L is a laboratory equipment designed for performing basic scientific tasks. It serves as a versatile tool for various laboratory applications. The core function of the PLO/L is to provide a controlled environment and support for sample handling and analysis.
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Lab products found in correlation
2 protocols using plo l
1
Feeder-free iPSC Differentiation to Neural Progenitors
2
Feeder-free iPSC Neural Induction
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Feeder-free iPSCs were dissociated with TryplE and seeded onto AggreWell800 plate (10,000 cells per EB; Stem Cell Technologies) in E8 medium supplemented with 10 μM ROCK inhibitor Y27632 for the first 24 hr; we changed 75% of the medium daily with STEMdiff NIM (Stem Cell Technologies). EBs were harvested after 5 days and plated onto poly-L-ornithine/laminin (PLO/L, Sigma)-coated plates. One to two days after attachment, prominent neural rosette structures were visible inside the attached neural aggregates. EBs were traced with ImageJ software. Rosettes were formed by cells expressing proteins characteristic of progenitor markers of PAX6 and Ki67.
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