ECM-producing HPSC cultures were fixed, permeabilized and processed as published (30 ). Samples were blocked using Odyssey® Blocking Buffer (LI-COR Biosciences, Lincoln, NE) containing 1% (v/v) donkey serum then incubated with either rabbit polyclonal anti-human fibronectin (1∶200) or 1A4 mouse anti-αSMA (1∶300), both from Sigma-Aldrich and diluted in blocking buffer. Fluorophore cross-linked secondary antibodies (Jackson ImmunoResearch Laboratories Inc.) were incubated at 1∶100 dilution. Samples were mounted using Prolong Gold anti-fading reagent from Life Technologies (Carlsbad, CA) and scanned using a confocal spinning disk Ultraview (Perkin-Elmer Life Sciences, Boston, MA) microscope. Images were acquired with a 60× 1.45 PlanApo TIRF oil immersion objective, under identical exposure conditions using Volocity 6.3.0 (Perkin-Elmer Life Sciences).
1a4 mouse anti αsma
Manufactured by Merck Group
The 1A4 mouse anti-αSMA is a lab equipment product produced by Merck Group. It is a monoclonal antibody that specifically binds to alpha smooth muscle actin (αSMA), a cytoskeletal protein found in smooth muscle cells. This product can be used to detect and quantify the presence of αSMA in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Volocity 6, by PerkinElmer (2 mentions)
Rabbit polyclonal anti human fibronectin, by Merck Group (2 mentions)
Fluorophore cross linked secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Odyssey blocking buffer, by LI COR (2 mentions)
Prolong gold antifading reagent, by Thermo Fisher Scientific (2 mentions)
2 protocols using 1a4 mouse anti αsma
1
Quantifying ECM Protein Expression in HPSCs
2
Quantifying ECM Protein Expression in HPSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ECM-producing HPSC cultures were fixed, permeabilized and processed as published (30 ). Samples were blocked using Odyssey® Blocking Buffer (LI-COR Biosciences, Lincoln, NE) containing 1% (v/v) donkey serum then incubated with either rabbit polyclonal anti-human fibronectin (1∶200) or 1A4 mouse anti-αSMA (1∶300), both from Sigma-Aldrich and diluted in blocking buffer. Fluorophore cross-linked secondary antibodies (Jackson ImmunoResearch Laboratories Inc.) were incubated at 1∶100 dilution. Samples were mounted using Prolong Gold anti-fading reagent from Life Technologies (Carlsbad, CA) and scanned using a confocal spinning disk Ultraview (Perkin-Elmer Life Sciences, Boston, MA) microscope. Images were acquired with a 60× 1.45 PlanApo TIRF oil immersion objective, under identical exposure conditions using Volocity 6.3.0 (Perkin-Elmer Life Sciences).
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