Nanoparticles were supplied from Lanxess Deutschland GmbH, Cologne (Germany), as latex suspension in water, with a concentration of around 30%wt. They have a dense core (around 48 ± 0.5 nm in diameter) with extended polymer chains protruding from the surface. The end groups of the chains have -OH functionalities. Polyacrylonitrile (PAN) average Mw 150 000, sodium metabisulphite, different dyes (Rose Bengal, Acid Fuchsin and Sunset Yellow), raffinose and magnesium sulfate (MgSO4) were purchased from Sigma Aldrich, UK, and used as received. Non-woven polypropylene fabric was purchased from Novatexx, Germany, (product code 2471). Poly(styrene-co-butadiene), with 45%wt styrene content, was purchased from Sigma Adrich, UK. Dimethylformamide (DMF) and toluene was purchased from VWR, UK.
Sunset yellow
Manufactured by Merck Group
Sourced in United States
Sunset Yellow is a laboratory reagent commonly used as a colorant in various scientific applications. It is a water-soluble, organic dye that produces a bright, yellow-orange hue. The core function of Sunset Yellow is to provide a distinct visual indicator or color marker for analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Allura red, by Merck Group (3 mentions)
Tartrazine, by Merck Group (3 mentions)
Quinoline yellow, by Merck Group (2 mentions)
Ponceau 4r, by Merck Group (2 mentions)
Acid fuchsin, by Merck Group (1 mentions)
Sodium metabisulphite, by Merck Group (1 mentions)
Rose bengal, by Merck Group (1 mentions)
Magnesium sulfate mgso4, by Merck Group (1 mentions)
Raffinose, by Merck Group (1 mentions)
Poly styrene co butadiene, by Merck Group (1 mentions)
Pgp glo assay system, by Promega (1 mentions)
Rhodamine 123 r123, by Merck Group (1 mentions)
Verapamil, by Promega (1 mentions)
Trans 4 hydroxy l proline trans hyp, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Hydroquinone, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
K4fe cn 6, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
8 protocols using sunset yellow
1
Nanoparticle-Coated Polypropylene Fabric
2
Pgp-Glo Assay for Transporter Inhibition
3
Chiral Doping of Sunset Yellow Liquid Crystal
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Sunset Yellow (SSY,
Sigma-Aldrich) was used as received. At room temperature, it is a
red powder and shows a nematic phase if dissolved in water above 28%
in weight. Chirality is induced doping the SSY with a proper amount
of trans-4-Hydroxy-l -proline (Trans-Hyp)
from Sigma-Aldrich. Paraffin oil and Span80 were purchased from Sigma-Aldrich.
A mixture of paraffin oil and Span80 was used as an oil matrix in
the microfluidic device. Nonchiral SSY liquid crystal mixtures were
prepared using deionized water (18.2 MΩ cm) to make a solution
of known concentration and phase (30% wt for SSY). For chiral mixtures,
we added 16% wt and 26% wt Trans-Hyp (dissolved in water) to 30% wt
SSY.
Sigma-Aldrich) was used as received. At room temperature, it is a
red powder and shows a nematic phase if dissolved in water above 28%
in weight. Chirality is induced doping the SSY with a proper amount
of trans-4-Hydroxy-
from Sigma-Aldrich. Paraffin oil and Span80 were purchased from Sigma-Aldrich.
A mixture of paraffin oil and Span80 was used as an oil matrix in
the microfluidic device. Nonchiral SSY liquid crystal mixtures were
prepared using deionized water (18.2 MΩ cm) to make a solution
of known concentration and phase (30% wt for SSY). For chiral mixtures,
we added 16% wt and 26% wt Trans-Hyp (dissolved in water) to 30% wt
SSY.
4
Evaluating Sunset Yellow and Allura Red Effects
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Thirty Wistar albino adult male rats of about 150 g body weight (b wt) were used in this work. Rats were kept in our animal facility for about 1 week before starting the experiment, for acclimatisation to the laboratory conditions.
Animals were housed in small plastic cages, maintained under constant conditions and were fed on a standard basal diet, as previously described in Khayyat et al. (2017) (link). Our experimental procedures were approved by the Menoufia University IACUC Committee for Care of Laboratory Animals (Approval No.: MNSP155).
Sunset Yellow (CAS 2783-94-0; 90% purity) and Allura Red (CAS 25956-17-6; 80% purity) were purchased from Sigma–Aldrich (Munich, Germany).
Animals were housed in small plastic cages, maintained under constant conditions and were fed on a standard basal diet, as previously described in Khayyat et al. (2017) (link). Our experimental procedures were approved by the Menoufia University IACUC Committee for Care of Laboratory Animals (Approval No.: MNSP155).
Sunset Yellow (CAS 2783-94-0; 90% purity) and Allura Red (CAS 25956-17-6; 80% purity) were purchased from Sigma–Aldrich (Munich, Germany).
5
Electrochemical Analysis of Antioxidants
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Ultrapure water was obtained using a Wasselab Purifier System. Methanol, phosphoric acid, NaH2PO4, and Na2HPO4 were obtained from Merck (Darmstadt, Germany). Chitosan (low molecular weight), 1-butil-3-metilimidazolio tetrafluoroborate, and K4Fe(CN)6 were obtained from Sigma–Aldrich (Milwaukee, WI, USA). The stock solutions, rutin, morin, quercetin, dopamine, ascorbic acid, uric acid, hydroquinone, tartrazine, and sunset yellow (Sigma–Aldrich) were prepared only once for the entire study (0.6 mmol L−1) in Methanol. The phosphate buffer solutions (PBS) as electrolyte were prepared in the pH range between 2.0 and 7.0 range using 0.010 mol L−1 phosphoric acid, sodium phosphate, and disodium phosphate solutions.
6
Colorimetric Dye Assay Protocol
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E. coli JM109, E. coli BL21 (DE3), and plasmid pET-22b (+) were deposited in our lab. Pyrobest DNA Polymerase, restriction enzymes, pMD18-T vector cloning Kit, Plasmid Mini Kit, T4 DNA ligase, Gel Extraction and Purification Kit, and DNA Extraction Kit were supplied by TAKARA Bio Inc. (Dalian, China). ABTS and synthetic dyes (azophloxine, etythrosine, Sunset Yellow, Ponceau 4R, Amaranth, Indigo Carmine, Acid Orange II, Congo Red) were ordered from Sigma-Aldrich (St. Louis, MO, USA).
7
Enzymatic Biosensor Development Protocol
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Laccase (E.C. 1.10.3.2) (0.5 µg, Sigma Aldrich, St. Louis, MO, USA), Sunset Yellow (98%, Sigma Aldrich), acrylamide (98%, Sigma Aldrich), ethyl methacrylate (97%, Sigma Aldrich), 2,2-dimethoxy-2-phenylacetophenone (99%, Sigma Aldrich), potassium dihydrogen phosphate (99%, Systerm, Shah Alam, Malaysia), alumina (Autolab, Ultrecht, The Netherlands), and dipotassium hydrogen phosphate (99%, Systerm) were used as received without further purification. The biosensor response was measured using a potentiostat (DropSens, Asturias, Spain) and GCE (Autolab).
8
Fluorescent Compound Preparation Protocol
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Urea and TEG, purchased from Merck (Darmstadt, Germany), were used to prepare the CDs. Quinine sulfate (Sigma-Aldrich, St. Louis, MO, USA) was used as the fluorescence standard compound. Sunset yellow, allura red, quinoline yellow, and tartrazine were all purchased from Sigma-Aldrich. The stock solutions of tartrazine and allura red were prepared in water. Ethanol was used to prepare the stock solutions of Sunset yellow and quinoline yellow. The working solutions were prepared by an appropriate dilution of the stock solution (1000 μM).
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