The Raw 264.7 mouse macrophage cell line was obtained from the Korean Cell Line Bank (KCLB; Seoul, Korea). Cells were cultured at 37°C under a humidified, 5% CO2 atmosphere in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% Fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL of streptomycin. In all experiments, cells were allowed to acclimate for 24 h before treatment. After reaching confluence, cells were incubated with GRo for 1 h at different concentrations up to 200μM followed by 1 μg/mL LPS for 24 h. In some experiments, Raw 264.7 cells were also pretreated with 10μM SnPP for 1 h prior to GRo and/or LPS treatment.
Raw264.7 mouse macrophage cell line
Manufactured by Korean Cell Line Bank
Sourced in United States
RAW264.7 is a mouse macrophage cell line. It is a type of immortalized cell line derived from Abelson murine leukemia virus-induced tumor cells. The RAW264.7 cell line is commonly used in research applications to study macrophage biology and function.
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Lab products found in correlation
Anti gapdh, by Thermo Fisher Scientific (1 mentions)
Anti phospho jnk, by Thermo Fisher Scientific (1 mentions)
Anti phospho p38 mapk, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Thermo Fisher Scientific (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Uv 550, by PerkinElmer (1 mentions)
Anti iκbα, by Thermo Fisher Scientific (1 mentions)
Anti p38 mapk, by Cell Signaling Technology (1 mentions)
Taqman probe, by Thermo Fisher Scientific (1 mentions)
Anti phospho stat3, by Cell Signaling Technology (1 mentions)
Griess reagent, by Merck Group (1 mentions)
Drx 500, by Bruker (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by GenDEPOT (1 mentions)
Ez cytox solution, by Daeil Lab Service (1 mentions)
Dulbecco modified eagle medium (dmem), by GenDEPOT (1 mentions)
4 protocols using raw264.7 mouse macrophage cell line
1
Macrophage Response to GRo and LPS
2
UV/IR Spectral and NMR Analysis
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The UV and IR spectra A were obtained using Jasco UV-550 and Perkin-Elmer model LE599 spectrometer, respectively. A Bruker DRX 500 or 700 MHz spectrometer were used for the analysis of NMR signals using methanol-d4 as solvents. ESIMS data were measured on VG Autospec Ultima Mass spectrometers.
A RAW264.7 mouse macrophage cell line was obtained from Korean Cell Line Bank (No. 40071, Seoul, Korea), and a HaCaT human keratinocyte cell line was obtained from Cell Lines Service GmbH (No. 300493, Eppelheim, Germany). Griess reagent was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). A TaqMan probe was obtained from Applied Biosystems (Foster City, CA, USA). To confirm the change in protein level, anti-IκB-α, anti-phospho-IκB-α, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK and anti-GAPDH were obtained from Invitrogen (Carlsbad, CA, USA) and anti-p38 MAPK, anti-phospho-p38 MAPK, anti-STAT3 and anti-phospho-STAT3 were purchased from Cell Signaling (Danvers, MA, USA).
A RAW264.7 mouse macrophage cell line was obtained from Korean Cell Line Bank (No. 40071, Seoul, Korea), and a HaCaT human keratinocyte cell line was obtained from Cell Lines Service GmbH (No. 300493, Eppelheim, Germany). Griess reagent was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). A TaqMan probe was obtained from Applied Biosystems (Foster City, CA, USA). To confirm the change in protein level, anti-IκB-α, anti-phospho-IκB-α, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK and anti-GAPDH were obtained from Invitrogen (Carlsbad, CA, USA) and anti-p38 MAPK, anti-phospho-p38 MAPK, anti-STAT3 and anti-phospho-STAT3 were purchased from Cell Signaling (Danvers, MA, USA).
3
RAW 264.7 Mouse Macrophage Cell Culture
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The RAW 264.7 mouse macrophage cell line, was obtained from Korea cell line bank (KCLB, Seoul, Republic of Korea). The composition of cell culture medium was DMEM containing 10% FBS and 1% penicillin-streptomycin [25 (link)]. RAW 264.7 cells were cultured in the complete medium at 37 °C in a humidified 5% CO2 incubator.
4
Cytotoxicity of Quinoa and Fermented Quinoa on RAW264.7 Macrophages
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RAW264.7 mouse macrophage cell line was purchased from Korean Cell Line Bank (Seoul, Korea) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gendepot, USA) supplemented with 10% (v/v) fetal bovine serum (FBS, Gendepot, USA), 100 U/mL penicillin and 100 µg/mL streptomycin (Invitrogen, USA) at 37 °C in 5% CO2 (Choi et al. 2018b (link); Maxwell et al. 2017 (link)). RAW264.7 macrophage cell was seeded on 96 wells plate at 2 × 104 cells/well and cultured for 48 h. Cells were rinsed with phosphate-buffered saline (PBS) and then treated with quinoa or fermented quinoa extract in DMEM medium without Fetal bovine serum (FBS) ranging from 1.56 to 1600 µg/mL obtained by diluting quinoa or fermented quinoa extract with the culture medium. RAW 246.7 cells cultured in a medium without adding samples were used as controls. After 24 h at 37 °C, 90 µL of medium was mixed with 10 µL of Ez-CyTox solution (Daeil Lab Service, Seoul, Korea) and then incubated at 37 °C for 1 h. Absorbance was measured at 450 nm using SpectraMax M3. Percent viability was calculated as cell viability relative to the control.
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