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1

Multimodal Immunolabeling of Diverse CNS Targets

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Catalogue numbers and concentrations of all antibodies are as follows. Anti-GFAP (130300, rat, 1:200), anti-occludin (711500, rabbit, 1:125) and anti-IgG (A11029, mouse, 1:100) were from Invitrogen. Anti–JAM-A (sc53623, mouse, 1:100) was from Santa Cruz Biotechnology. Fluoromyelin was from ThermoFisher (F34651, 1:300). Anti-fibrinogen (A0080, rabbit, 1:150) was from Dako. Anti-CD3 (16-0037-85, mouse, 1:100), anti-CD4 (14-9766-82, rat, 1:100), Anti-CD31 (550274, rat, 1:100), anti-CD45 (550539, rat, 1:100) were from eBioscience. anti-CD4 (ab183685, mouse, 1:50) was from Abcam. Anti-NeuN (MAB377, mouse, 1:100), anti-Olig2 (MABN50, mouse, 1:500) and anti-AQP4 (AB3594, rabbit, 1:200) were from Millipore. Anti-laminin (L9393, rabbit, 1:200) was from Sigma–Aldrich. Anti-Iba1 (109-19741, rabbit, 1:500) was from Wako.
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2

Antibody Sources and Reagents

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Antibodies were obtained from the following commercial sources: anti-NOXA (Calbiochem, Gibbstown, NJ), anti-tubulin (Sigma-Aldrich, St. Louis, MO) and anti-JAM-A (Santa Cruz Biotechnology, Santa Cruz, CA for flow cytometry, abcam, Cambridge, MA for immunoblotting). Reolysin was provided by Oncolytics Biotech Inc. (Calgary, AB). Bortezomib (VelcadeTM) was obtained from Millennium Pharmaceuticals.
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