Exosomes in the plasma of patients and cell culture were isolated according to the previous study [17 ]with slight modification. In brief, exosomes were isolated by differential centrifugation and the size of which was analyzed by a nanoparticle tracking analysis (NTA) using a Zetasizer Nano S90 system (Malvern Instruments, England). Exosome morphology was observed using a transmission electron microscope (TEM) (JEM-1200EX; JEOL, Tokyo, Japan). Exosome was identified using markers of protein as CD63, TSG101 and HSP70 in the exosomes pellet fraction assayed by Western blot.
Zetasizer nano s90 system
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Nano S90 system is a dynamic light scattering (DLS) instrument designed for the measurement of particle size and zeta potential. It is capable of determining the size of particles or molecules in the range of 0.3 nm to 10 μm and zeta potential measurements in the range of ±500 mV.
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Lab products found in correlation
3 protocols using zetasizer nano s90 system
1
Exosome Isolation and Characterization
2
Exosome Characterization by NTA and TEM
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Exosomes were isolated by differential centrifugation. The exosome size and number were measured by a nanoparticle tracking analysis (NTA) using a Zetasizer Nano S90 system (Malvern Instruments, England) equipped with a blue laser (405 nm). Purified exosomes were observed with a JEM1400 transmission electron microscope (TEM) operated at 120 kV (JEOL, Japan).
3
Characterizing Nanoparticle Size and Charge
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A Malvern Zetasizer Nano S90 system was used to measure the electrophoretic mobility and DLS of DNFs. Laser Doppler velocimetry was performed to determine the zeta potential for assessing the electrophoretic mobility of charged particles. In DLS measurements, the speed of Brownian motion depends on the particle size and viscosity of the medium. The Zetasizer software was used to calculate the size of DNF particles, which reflects the average hydrodynamic diameter and particle strength. The values from three runs are presented as the average ± standard deviation.
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