Anti stat3 antibody 79d7

DU145 cells were treated with 2D5 peptide at the indicated concentrations and for the indicated periods and then lysed in lysis buffer (20 mM Tris–HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% deoxycholate, and 0.1% SDS) with sonication. After centrifugation, the supernatants were subjected to SDS-PAGE and then immunoblotted. The following antibodies were used for immunoblotting: anti-Myc antibody (9E10, Sigma), anti-HA antibody (C29F4, Cell Signaling Technology), anti-EGFR antibody (D38B1, Cell Signaling Technology), anti-phospho-EGFR Y1068 antibody (Cell Signaling Technology), anti-GST antibody (Z-5, Santa Cruz Biotechnology), anti-STAT3 antibody (79D7, Cell Signaling Technology), anti-phospho-STAT3 antibody (GeneTex), anti-ERK antibody (K-23, Santa Cruz Biotechnology), anti-phospho-ERK antibody (Cell Signaling Technology), anti-β-actin antibody (AC-15, Santa Cruz Biotechnology), anti-p65 antibody (D14E12, Cell Signaling Technology), anti-phospho-p65 antibody (93H1, Cell Signaling Technology), and anti-phosphotyrosine antibody (4G10, GeneTex).

ChIP was performed using the Magna ChIP A/G kit (EMD Millipore, Burlington, MA, USA) following manufacturer’s recommendations, and quantified by qPCR. Anti-STAT3 antibody (79D7) used in ChIP experiment was purchased from Cell Signaling Technology (Danvers, MA, USA). Table S3 lists the specific primers used for ChIP-qPCR.