Nbp1 69750

Manufactured by Novus Biologicals

NBP1-69750 is a laboratory product manufactured by Novus Biologicals. It is a tool used for research purposes, but a detailed description is not available while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

A2052, by Merck Group (1 mentions) Ab5603, by Merck Group (1 mentions) Ab3905, by Abcam (1 mentions) Mab377, by Merck Group (1 mentions) Ab144p, by Merck Group (1 mentions) Mab369, by Merck Group (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) Ab18465, by Abcam (1 mentions) Sc 8066, by Santa Cruz Biotechnology (1 mentions) Ab112, by Abcam (1 mentions)

2 protocols using nbp1 69750

Immunohistochemistry of Brain Sections

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical analysis of 40-μm brain sections were conducted essentially as described previously (Niu et al., 2013 (link), Niu et al., 2015 (link)). The following primary antibodies were used: BrdU (rat BU1/75, 1:500, Accurate Chemical), NeuN (MAB377, mouse, 1:500, Millipore), SOX2 (AB5603, rabbit, 1:500, Millipore), DCX (SC-8066, goat, 1:150, Santa Cruz Biotechnology), TH (chick, 1:1,000, Aves), DARPP32 (no. 2302, rabbit, 1:500, Cell Signaling Technology), CTIP2 (AB18465, rat, 1:500, Abcam), DAT (MAB369, rat, 1:200, Millipore), DDC (Ab3905, rabbit, 1:500, Abcam), VMAT2 (NBP1-69750, rabbit, 1:250, Novus), VGLUT1 (135311, mouse, 1:1,000, Synaptic), CHAT (AB144P, goat, 1:200, Chemicon), and GABA (A2052, rabbit, 1:500, Sigma). Alexa Fluor 488-, 594-, or 647-conjugated corresponding secondary antibodies from Jackson ImmunoResearch were used for indirect fluorescence. Images were taken using a Zeiss LSM510 confocal microscope. A Cell Counter software plugin in the ImageJ program was used to count cells. Data were obtained from one-sixth of the sections spanning the whole striatal region in each mouse. A representative image was shown from at least four similar ones. Confocal images were Z series projections unless otherwise indicated in the figure legends.

Niu W., Zang T., Wang L.L., Zou Y, & Zhang C.L. (2018). Phenotypic Reprogramming of Striatal Neurons into Dopaminergic Neuron-like Cells in the Adult Mouse Brain. Stem Cell Reports, 11(5), 1156-1170.

+ Open protocol

+ Expand

Immunohistochemical Labeling of Neurological Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin-embedded human brain sections or free-floating mouse brain sections sliced by cryotome were treated with 0.3% H₂O₂ for 10 min. Sections were washed three times in PBS and blocked in 1% BSA, 0.3% Triton X-100, for 1 h, followed by overnight incubation with a tyrosine hydroxylase (TH) antibody (Abcam, AB112; 1: 1000), anti-T22, anti-Tau N368, anti-ApoE, anti-AEP, anti-VMAT2 (Novus, NBP1-69750), or AT-8 antibody (1: 500) at 4°C. Then, the sections were incubated with the matched fluoro-conjugated secondary antibody for 2 h at room temperature, followed by three washes in PBS. The slides were washed three times in PBS and covered with a glass using mounting solution, after DAPI staining for 5 min. Human brain sections were treated with 10 mM cupric sulfate in ammonium acetate buffer (50 mM) for 15 min to eliminate lipofuscin-like auto fluorescence.

Kang S.S., Ahn E.H., Liu X., Bryson M., Miller G.W., Weinshenker D, & Ye K. (2021). ApoE4 Inhibition of VMAT2 in the Locus Coeruleus Exacerbates Tau Pathology in Alzheimer’s Disease. Acta neuropathologica, 142(1), 139-158.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!