The UCNP solutions were excited by a 980 nm continuous-wave (CW) single-mode diode laser (P161-600-980A, EM4, Andor Technology, Belfast, UK). Their emission was collected through an optical fibre by a home-made UCL spectrograph and imaging system, which was composed of an inverted microscope (IX71, Olympus, Tokyo, Japan) and an electron multiplying charge coupled device (EMCCD) camera (DV897DCS-BV, iXon, Andor Technology, Belfast, UK). It was detected by a CCD camera (PIXIS 400BR, Princeton Instruments, Trenton, NJ, USA) attached to an imaging spectrograph (IsoPlane SCT320, Princeton Instruments). The images of individual UCNPs or UCNPs in sliced SLN tissues were taken from the same imaging system. To obtain colour images of SLN tissues, a colour sCMOS camera (OS4MPc-CL-RGB, Raptor Photonics, Larne, UK) was employed.
Dv897dcs bv
Manufactured by Oxford Instruments
Sourced in United Kingdom
The DV897DCS-BV is a high-performance digital voltmeter designed for laboratory and industrial applications. It offers accurate and reliable voltage measurements across a wide range of scales, providing users with the necessary tools to effectively monitor and analyze electrical signals in their work environment.
Automatically generated - may contain errors
2 protocols using dv897dcs bv
1
Imaging Upconversion Nanoparticles in Lymph Nodes
2
In Vivo and Ex Vivo Imaging of iRFP713 Fluorescence
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To detect iRFP713 near-infrared fluorescence in vivo, the mice were anesthetized with 2–3% isoflurane (Isothesia, Henry Schein Vet Pharma). Fur was removed with a commercial electrical razor (Veet Sensitive Prescision) and depilatory cream (Veet) in regions of interest. Excitation of iRFP713 was enabled by a 670 nm diode laser (300 mW, BWF1-670-300E, B&W Tek, Inc.), while fluorescence emission at 740/40 nm (ET740/40x, Chroma Technology) was detected in epi-illumination mode by iXon electron multiplying charge-coupled device (EMCCD, DV897DCS-BV, Andor Technology).
For iRFP713 imaging ex vivo, organs and tissues were dissected, placed in a 12-well cell culture plate (Sarstedt), and detected using an infrared fluorescence imaging system (Odyssey Infrared Imaging System, LI-COR Biosciences GmbH). With 700 nm laser excitation, near-infrared fluorescence emission from tissue was visualized.
For iRFP713 imaging ex vivo, organs and tissues were dissected, placed in a 12-well cell culture plate (Sarstedt), and detected using an infrared fluorescence imaging system (Odyssey Infrared Imaging System, LI-COR Biosciences GmbH). With 700 nm laser excitation, near-infrared fluorescence emission from tissue was visualized.
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