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4 protocols using pyrene

1

Synthesis of Fluorescent Gold Nanoparticles

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The α- and β-CDs, phosphate buffer solution (PBS, 0.1 M, pH = 7.0), pyrene and R6G were purchased from FUJIFILM Wako Pure Chemical Corporation. Chloroauric acid (HAuCl4·4H2O) and ethanol were obtained from Kishida Chemical Corporation. 4-Aminothiophenol (4-ATP) was procured from Tokyo Chemical Industries. All experiments were carried out using ultrapure water (>18 MΩ cm) prepared with a Direct-Q water purification system.
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2

Synthesis and Characterization of Ionic Polymers

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2-Acrylamido-2-methylpropanesulfonic acid (AMPS, 98%, Tokyo Chemical Industry, Tokyo, Japan), 4,4′-azobis(4-cyanovaleric acid) (V-501, 98%, Wako Pure Chemical, Osaka, Japan), tetrabutylphosphonium bromide (P4444Br, 95%, Wako Pure Chemical), and tributyl-n-octylphosphonium bromide (P4448Br, 98%, Tokyo Chemical Industry) were used as received. 4-Cyanopentanoic acid dithiobenzoate (CPD) was synthesized as previously reported [18 (link)]. Pyrene (97%, Wako Pure Chemical) was purified by recrystallization from ethanol. Methanol was dried with 3A molecular sieves and purified by distillation. Water was purified with an ion exchange column system. Other reagents were used as received.
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3

Synthesis and Characterization of PEO-Br Macroinitiator

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Tris(2-(dimethylamino)ethyl)amine (Me6TREN, 97%) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Copper bromide (CuBr, 95.0%) was supplied by Kishida Chemical (Osaka, Japan). Ethanol (99.5%), tetrahydrofuran (THF, 99.5%), and cetylpyridinium chloride (90%) were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). All chemicals were used as received; 2-Methoxyethyl acrylate (MEA, 98.0%) from Fujifilm Wako Pure Chemical (Osaka, Japan) was treated with an inhibitor-remover prepacked column from Sigma-Aldrich (St. Louis, MO, USA) prior to use. PEO (MW = 500,000 g/mol) was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). PEO-based macroinitiator (PEO-Br) was prepared and purified in accordance with the literature [28 (link)]. Number average molecular weight (Mn(GPC)) and molecular weight distribution (Mw/Mn) estimated from gel-permeation chromatography (GPC) for PEO-Br were 4.64 × 105 g/mol and 1.23, respectively. The degree of polymerization (DP) for PEO-Br was 11,340. Pyrene (97%) from Fujifilm Wako Pure Chemical (Osaka, Japan) was recrystallized from mEthanol. Water was purified using an ion-exchange column system.
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4

Synthesis and Characterization of Aromatic Compounds

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Section 1: naphthalene (Sigma-Aldrich, St. Louis, MO, USA), anthracene (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), tetracene (Tokyo Chemical Company (TCI), Tokyo, Japan), pyrene (Wako).
Section 2: 9,10-diphenylanthracene (Sigma-Aldrich), 9,10-bis(phenylethynyl)anthracene (TCI), 2,7-di-tert-butyllpyrene (TCI), 1,3,6,8-tetraphenylpyrene (TCI), 5,12-bis(phenylethynyl)tetracene (Sigma-Aldrich).
Section 3: D-/L-camphor (Sigma-Aldrich), (R)-/(S)-(-)-1,1′-binaphthyl-2,2′-diyl hydrogen phosphate (TCI), (R)-/(S)-2,2′-dimethoxy-1,1′-binaphthyl (TCI), (R)-/(S)-2,2′-bis(methoxymethoxy)- 1,1’-binaphthyl (TCI).
Section 4: biphenyl (TCI), p-terphenyl (TCI), p-quaterphenyl (TCI), p-quinquphenyl (TCI), p-hexaphenyl (TCI), DMT (Exciton, Tokyo Instruments Inc (Tokyo, Japan)), Japanese vendor)), TMQ (Exciton), QUI (Exciton), Exalite 360 (Exciton), TBS (Exciton), [12 (link)] cycloparaphenylene (Kanto Chemicals, Tokyo, Japan).
Section 5: 9,9-dimethylfluorene (TCI), 9,9-dioctylfluorene (TCI), Exalite384 (Exciton), 9,9-dihexylfluorene trimer (American Dye Source (ADS), Quebec, Canada), 9,9-dihexylfluorene pentamer (ADS), 9,9-dihexylfluorene heptameter (ADS), Exalite 376 (Exciton), Exalite 404 (Exciton), Exalite 428 (Exciton), Exalite 416 (Exciton).
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