Aav1 cag radcyap1r1 shrnamir gfp

Ethanol solution (10% w/v) was prepared using 95% ethyl-alcohol and tap water. Saccharin solution (0.02% w/v) was prepared using Saccharin sodium salt hydrate (Sigma Aldrich, St. Louis, MI) and tap water. Sucrose solution (1.5% training, 5% w/v maintenance and testing) was prepared using Sucrose (Sigma Aldrich, St. Louis, MI) and tap water. PACAP(6–38) was purchased from the American Peptide Company (Sunnyvale, CA). The peptide was dissolved in sterile isotonic saline in the presence of 1% bovine serum albumin (Sigma Aldrich, St. Louis, MI). A PAC1R short-hairpin knockdown adeno-associated virus (AAV1-CAG-rADCYAP1R1-shRNAmir-GFP, Vigene Biosciences, Rockville, MD, “AAV-PAC1R-KD”), and a control virus (AAV1-CAG-GFP, Addgene, Watertown, MA, “AAV-CTRL”) were used for the NAcc Core Adcyap1r1 gene (i.e. PAC1R) knockdown experiment.