Mutant versions of the miR-146a-5p binding site sequences in JAZF1 were synthesized and inserted into the psiCHECK2 luciferase reporter vector (Hanbio Biotechnology) to create JAZF1 wild-type (JAZF1-WT) and mutant (JAZF1-Mut). The dual-luciferase assay was conducted using a dual-luciferase assay kit (Hanbio Biotechnology). The relative luciferase activity of fireflies and kidney worms was measured using SpectraMax M5e.
Psicheck2 luciferase reporter vector
Manufactured by Hanbio Biotechnology
Sourced in China
The PsiCHECK2 luciferase reporter vector is a dual-luciferase reporter system used for monitoring gene expression in eukaryotic cells. It contains both Renilla and Firefly luciferase reporter genes, allowing for the simultaneous measurement of two different reporter activities within a single sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using psicheck2 luciferase reporter vector
1
Validation of miR-146a-5p Binding Site
2
Luciferase Reporter Assay for miR-16-5p Binding
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The sequences of DLX6-AS1 and BMI1 3'UTR harboring wide-type (WT) or mutant-type (MUT) miR-16-5p binding fragments were introduced into psiCHECK-2 luciferase reporter vector (Hanbio Biotechnology Co., Ltd., Shanghai, China), and named DLX6-AS1WT, DLX6-AS1 MUT, BMI1 3'UTR-WT, or BMI1 3'UTR-MUT reporter. These reporters together with miR-16-5p mimic or miR-NC were introduced into A549 and H1299 cells for 48 h, luciferase activities were discovered using the dual-luciferase reporter assay kit (Promega, Madison, WI, USA).
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