Fcf 400 ni

Manufactured by Electron Microscopy Sciences

Sourced in United States

The FCF-400-Ni is a core-shell fluorescent carbon fiber (FCF) product manufactured by Electron Microscopy Sciences. It is designed for use in a variety of applications that require a durable and highly fluorescent material.

Automatically generated - may contain errors

Lab products found in correlation

Morgagni 268d, by Philips (2 mentions) Tecnai g20 cryo, by Thermo Fisher Scientific (1 mentions)

2 protocols using fcf 400 ni

Electron Microscopy Sample Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The preparation of samples for electron microscopy was previously described (Boyer et al., 2011 (link)). Briefly, samples were fixed with glutaraldehyde (2.5%) and cacodylate buffer (0.1 M), cut into 70-nm sections using an ultramicrotome (UC7; Leica), collected on 400-mesh nickel grids with formvar carbon, and stained for electron microscopy (FCF-400-Ni, Electron Microscopy Sciences). The samples were then viewed with a Philips electron microscope (Morgagni 268D) at 80 keV. Cross sections of all pictures that were selected for the analysis were positioned at the middle of the virions and characterized by a dense cluster (black mass).

Sobhy H., Scola B.L., Pagnier I., Raoult D, & Colson P. (2015). Identification of giant Mimivirus protein functions using RNA interference. Frontiers in Microbiology, 6, 345.

+ Open protocol

+ Expand

Negative Staining for Cellular Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Negative staining was performed in order to observe the cells' morphology. The cells were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer for at least 1 hour at 4°C. A drop of cell suspension was deposited for approximately 5 minutes on glow-discharged Formvar carbon film with 400 mesh nickel grids (FCF400-Ni; Electron Microscopy Sciences (EMS), Hatfield, PA, USA). The grids were dried on blotting paper, and the cells were negatively stained for 10 seconds with 1% ammonium molybdate solution in filtered water at room temperature. Electron micrographs were acquired with a Tecnai G20 Cryo (FEI Company, Limeil-Brévannes, France) transmission electron microscope operated at 200 keV or with a Morgagni 268D (Philips, Amsterdam, The Netherlands) transmission electron microscope operated at 80 keV.

Andrieu C., Mailhe M., Ricaboni D., Fonkou M.D., Bilen M., Cadoret F., Tomei E., Armstrong N., Vitton V., Benezech A., Davoust B., Levasseur A., Lagier J.C., Fournier P.E, & Raoult D. (2018). Noncontiguous finished genome sequences and description of Bacteroides mediterraneensis sp. nov., Bacteroides ihuae sp. nov., Bacteroides togonis sp. nov., Bacteroides ndongoniae sp. nov., Bacteroides ilei sp. nov. and Bacteroides congonensis sp. nov. identified by culturomics. New Microbes and New Infections, 26, 73-88.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!