Commercially available human chondrocytes (Provitro®, Berlin, Germany) were cultured in Chondrocyte Growth Medium basal (CGM, Provitro®, Berlin, Germany) supplemented with 10% fetal calf serum (Provitro®, Berlin, Germany), 100 IU penicillin/mL and 100 μg streptomycin/mL (Provitro®, Berlin, Germany).
Penicillin
Manufactured by Provitro
Sourced in Germany
The Penicillin lab equipment is designed for the cultivation and production of the antibiotic penicillin. It provides a controlled environment for the growth and fermentation of the penicillin-producing microorganisms. The core function of this equipment is to facilitate the production and extraction of the penicillin substance.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Provitro (6 mentions)
Fetal calf serum (fcs), by Provitro (3 mentions)
Human chondrocytes, by Provitro (2 mentions)
Igf 1, by Provitro (1 mentions)
L glutamine, by Provitro (1 mentions)
L glutamine, by Euroclone (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Streptomycin, by Corning (1 mentions)
Penicillin, by Corning (1 mentions)
Endothelial cell growth supplement (ecgs), by Corning (1 mentions)
Penicillin, by GE Healthcare (1 mentions)
Streptomycin, by GE Healthcare (1 mentions)
Dulbecco modified eagle medium (dmem), by Harvard Bioscience (1 mentions)
Supplement mix, by Provitro (1 mentions)
Skeletal muscle growth medium, by Provitro (1 mentions)
Amphotericin, by Provitro (1 mentions)
Streptomycin, by Harvard Bioscience (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
6 protocols using penicillin
1
Chondrocyte Culture and Expansion
2
Neuroblastoma Cell Culture and Differentiation
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Neuroblastoma cells (7 × 103) were plated for 2 days in the 'maintenance medium', as follows: hepatocyte defined medium, serum free (BD Biocoat, Bedford, MA, USA, cat. no. 05449), Human EGF (epidermal growth factor) 5 μg/ml (BD Biosciences, Bedford, MA, USA, cat. no. 354052); 2 mM L-Glutamine (Euroclone, Pero (MI), Italy, cat. no. ECB3000D), 1% penicillin (100 units/ml)/streptomycin (100 mg/ml) (Lonza, cat. no. DE17-602E), and 10 ng/ml IGF-1 (insulin growth factor 1) (Provitro, Berlin, Germany, cat. no. 1480950020). After 2 days, the cells were plated in 'differentiation medium': hepatocyte defined medium, serum free, EGF 5 μg/ml, 2 mM L-Glutamine, 1% penicillin (100 units/ml)/streptomycin (100 mg/ml), human HGF (hepatocyte growth factor) 20 ng/ml (Provitro, cat. no. 1468954010), 20 ng/ml FGF-4 (fibroblast growth factor 4) (Provitro, cat. no. 1372 9500 05). The culture medium was added every 2 days. After about 20 days, the cells were collected and used for the experiments.
3
Culturing Diverse Cell Lines for Experiments
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HEK293T, MCF7, and C2C12 cells were grown in DMEM (Biochrom AG) supplemented with 10% fetal calf serum (FCS; Biochrom AG), 2 mM l -glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) (PAA Laboratories) at 37°C and 10% (C2C12) or 5% CO2. Immortalized human myoblasts were cultured in skeletal muscle growth medium (Provitro) supplemented with supplement mix (Provitro), 50 ng/ml amphotericin, 50 µg/ml gentamicin, 10% FCS, 2 mM l -glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) at 37°C and 5% CO2. hFOBs (1.19) were cultured in a 1:1 mixture of DMEM and Ham’s F12 medium supplemented with 10% FCS, 2 mM l -glutamine, penicillin (100 U/ml)/streptomycin (10 µg/ml), and 0.3 mg/ml G418 (Biochrom AG) at 34°C with 5% CO2 to keep them in a proliferative state. HUVECs were a kind gift from M. Lorenz and V. Stangl (Charité Universitätsmedizin, Berlin, Germany) and cultured on gelatin-coated tissue culture ware in M199 medium supplemented with 20% FCS, 50 µg/ml endothelial cell growth supplement (Corning), 25 µg/ml heparin, 2 mM l -glutamine, and penicillin (100 U/ml)/streptomycin (10 µg/ml) at 37°C and 5% CO2. HUVECs were used at passage 3 in all experiments. Unless stated otherwise, all cells were starved for 5 h prior to stimulation with their respective growth medium, without FCS supplement, containing 2 mM l -glutamine and penicillin/streptomycin.
4
Culturing Patient-Derived Glioblastoma Stem Cells
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Patient-derived glioblastoma stem-like cultures (NCH644 and NCH601) were kindly provided by Dr. Christel Herold-Mende (University of Heidelberg, Germany) and cultured as previously described (Campos et al., 2010 (link); Sanzey et al., 2015 (link)). In brief, NCH644 cells were maintained in neurobasal medium (Invitrogen, Carlsbad, California, USA) supplemented with 2% (v/v) ultraglutamine (Lonza, Basel, Switzerland), 1x B27 supplement (Lifetech, Carlsbad, California, USA), 1 U/ml heparin (Sigma Aldrich, St. Louis, Missouri, USA), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Sigma Aldrich), 20 ng/ml epidermal growth factor (EGF) (Provitro, Berlin, Germany) and 20 ng/ml basic fibroblast growth factor (bFGF) (Milteny, Bergisch Gladbach, Germany). NCH601 cells were maintained in DMEM-F12 medium (Lonza) supplemented with 2% (v/v) ultraglutamine, 1x BIT100 supplement (Provitro), 1 U/ml heparin, 100 U/ml penicillin and 0.1 mg/ml streptomycin, 20 ng/ml EGF and 20 ng/ml bFGF. The cells were cultured in a humidified atmosphere at 5% CO2 and 37 °C and routinely checked for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). The spheroids morphology was estimated with EVOS fl AMF-4306 AMG microscope (Westburg, Leusden, Netherlands).
5
Chondrocyte Isolation and Culture
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Human chondrocytes from six different donors were bought from the company Provitro (Berlin, Germany). The cells were cultured in Chondrocyte Growth Medium (CGM; Provitro®, Berlin, Germany) supplemented with 10 % fetal calf serum (Provitro®, Berlin, Germany) and antibiotics -100 IU penicillin/ mL and 100 µg streptomycin/mL (Provitro®, Berlin, Germany). Chondrocytes of passage 3 grown in T25 cm 2 flasks (25 cm 2 ; Sarstedt, Nümbrecht, Germany) were used for the experiments. The procedure was described in detail in [6] .
For immunohistochemical studies, the cells were seeded in slide flasks (Thermo Fisher Scientific, Waltham, MA, USA) 24 h before the experiments (n=40 each group).
For immunohistochemical studies, the cells were seeded in slide flasks (Thermo Fisher Scientific, Waltham, MA, USA) 24 h before the experiments (n=40 each group).
6
Chondrocyte Culture and Preparation
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Human chondrocytes derived from hip joint cartilage were purchased from Provitro (Berlin, Germany). They were cultured in Chondrocyte Growth Medium (CGM; Provitro®, Berlin, Germany) supplemented with 10 % fetal calf serum (Provitro®, Berlin, Germany) and antibiotics -100 IU penicillin/mL and 100 μg streptomycin/mL (Provitro®, Berlin, Germany). The cells from frozen stocks (passage 1) were grown in T175 cell culture flasks (175 cm 2 ; Sarstedt, Nümbrecht, Germany) until sub-confluent layers were obtained. Afterwards, the cells were split (passage 2) in 5 T175 cell culture flasks and after reaching confluence 4 T175 were split (passage 3) in 80 T25 cell culture flasks (25 cm 2 ; Sarstedt, Nümbrecht, Germany) for the vibration experiments (n=40 for vibrated samples and n= 40 as 1g-control samples).
For histological investigations, the chondrocytes were seeded in slide flasks one day before the experiments (n=40 each group; Thermo Fisher Scientific, Waltham, MA, USA).
For histological investigations, the chondrocytes were seeded in slide flasks one day before the experiments (n=40 each group; Thermo Fisher Scientific, Waltham, MA, USA).
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