Decahydronaphthalene decalin

Tetramethoxysilane (TMOS), polyethylene glycol (PEG) (10,000 MW), ethanol, acetic acid, phosphoric acid, ammonium hydroxide, citric acid, sodium hydrosulfide, sulfuric acid, sodium citrate, sodium phosphate dibasic, phosphate buffer saline (PBS), bovine serum albumin (BSA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), human IgG, (3-Glycidyloxypropyl)trimethoxysilane (GPTMS), Tween 20, sucrose, tris buffer, and hydroquinone were purchased from Sigma-Aldrich (St. Louis, MO). Decahydronaphthalene (decalin) and N-(γ-maleimidobutyryloxy) sulfosuccinimidyl ester (GMBS), anti-human IgG (rabbit host) were purchased from Thermo Fisher Scientific (Rockford, IL). 5 nm gold nanoparticle (AuNPs) tagged anti-human IgG and anti-rabbit IgG were purchased from Cytodiagnostics (Burlington, ON). Silver acetate was purchased from Carolina Biological Supply Co. (Burlington, NC). Microfluidic fittings were purchased from IDEX Health & Science (Oak Harbor, WA) and needle tubing was purchased from Hamilton Syringe (Reno, NV). Capillary tubing with 360 μm O.D. and 250 μm I.D. was purchased from Polymicro Technologies (Phoenix, AZ).

Sodium citrate, ethanol, hydroquinone, phosphate buffer saline (PBS), bovine serum albumin (BSA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Tween 20, (3-Aminopropyl) triethoxysilane (APTES), and human IgG were purchased from Sigma-Aldrich (St. Louis, MO). Bis (sulfosuccinimidyl) suberate (BS3), and decahydronaphthalene (decalin) were purchased from Thermo Fisher Scientific (Rockford, IL). NR1-3000PY negative photoresist and RD-6 developer were purchased from Futurrex Inc. (Franklin, NJ). Silver enhancement solution, anti-human IgG (rabbit host), and 40 nm AuNPs tagged with anti-rabbit IgG were purchased from Cytodiagnostics (Burlington, ON). Silver acetate was purchased from Carolina Biological Supply Co. (Burlington, NC). Silica beads (20 µm diameter) were purchased from Corpuscular Inc. (Cold Spring, NY). Microfluidic fittings were purchased from IDEX Health & Science (Oak Harbor, WA) and needle tubing was purchased from Hamilton Syringe (Reno, NV).