Ang 1 8

Ang-[1–10], Ang-[1–8], Ang-[1–7], captopril (Cap), losartan, inactin, L-phenylephrine and Evans blue dye were purchased from Sigma (St. Louis, MO). Isoflurane was purchased from Cristália Ltda (Itapira, SP, Brazil). Ketoprofen (Ketoflex) was purchased from Mundo Animal (Pindamonhangaba, SP, Brazil). Penicillin, streptomycin, and dihydrostreptomycin were purchased from Fort Dodge Animal Health (Hialeah, FL, USA). Abz-Phe-Arg-Lys(Dnp)-Pro-OH was purchased from GenScript (Piscataway, NJ, USA). Mca-Ala-Pro-Lys(Dnp)-OH was purchased from Enzo Life Science (Farmingdale, NY, USA). MLN-4760 was purchased from EMD Milllipore (Billerica, MA, USA). Ceramic microbeads were purchased from MP Biomedicals (Solon, OH, USA). RNeasy was purchased from Qiagen (Germantown, MD, USA). RNase-free water, SYBR green supermix, and high-capacity cDNA reverse transcription kits (#1708890) were purchased from Bio-Rad (Hercules, CA, USA). Renin activity 520 Rat Renin Fluorimetric assay kit was purchased from SensoLyte (AnaSpec, San Jose-CA). Angiotensinogen Elisa kit was purchased from IBL (Hamburg, Germany). Aldosterone Elisa kit was purchased from Enzo Life Science (Farmingdale, NY, USA).